Abstract
The purpose of this study was to explore the effects of changes in Type I collagen on the viscoelasticity of bone. Bone coupons were heated at either 100 or 200 °C to induce the thermal denaturation of Type I collagen. Half of these specimens were rehydrated after heat treatment; the other half were tested in a dry condition. The degree of denatured collagen (DC%) was analyzed by a selective digestion technique with the use of α-chymotrypsin. Isothermal (37 °C) and variable temperature tests (scans from 35 to 200 °C) were performed with the use of a dynamic mechanical analyzer to evaluate changes in bone viscoelastic properties as a function of collagen damage, specifically, changes in the loss factor (tan δ) and storage modulus (E′) were assessed. Significant collagen denaturation occurred only when bone was heated at 200 °C irrespective of the hydration condition. Also, DC% did not show a significant effect on tan δ. However, higher values of tan δ were observed in wet samples compared to dry specimens. The temperature-scan tests revealed that the hydration condition, but not DC%, significantly affected the behavior of tan δ. However, E′ was not strongly influenced either by DC% or by water content. These results suggest that at a constant frequency the denaturation of collagen triple-helical molecules may have few effects on the viscoelasticity of bone, but moisture may play a prominent role in determining this property.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 31-36 |
| Number of pages | 6 |
| Journal | Journal of Biomedical Materials Research |
| Volume | 63 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2002 |
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Keywords
- Collagen denaturation
- Cortical bone
- Loss tangent
- Moisture
- Viscoelasticity
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
Cite this
Collagen and bone viscoelasticity : A dynamic mechanical analysis. / Yamashita, Junro; Li, Xiaoe; Furman, Benjamin R.; Rawls, Henry R; Wang, Xiaodu; Mauli Agrawal, C.
In: Journal of Biomedical Materials Research, Vol. 63, No. 1, 2002, p. 31-36.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Collagen and bone viscoelasticity
T2 - A dynamic mechanical analysis
AU - Yamashita, Junro
AU - Li, Xiaoe
AU - Furman, Benjamin R.
AU - Rawls, Henry R
AU - Wang, Xiaodu
AU - Mauli Agrawal, C.
PY - 2002
Y1 - 2002
N2 - The purpose of this study was to explore the effects of changes in Type I collagen on the viscoelasticity of bone. Bone coupons were heated at either 100 or 200 °C to induce the thermal denaturation of Type I collagen. Half of these specimens were rehydrated after heat treatment; the other half were tested in a dry condition. The degree of denatured collagen (DC%) was analyzed by a selective digestion technique with the use of α-chymotrypsin. Isothermal (37 °C) and variable temperature tests (scans from 35 to 200 °C) were performed with the use of a dynamic mechanical analyzer to evaluate changes in bone viscoelastic properties as a function of collagen damage, specifically, changes in the loss factor (tan δ) and storage modulus (E′) were assessed. Significant collagen denaturation occurred only when bone was heated at 200 °C irrespective of the hydration condition. Also, DC% did not show a significant effect on tan δ. However, higher values of tan δ were observed in wet samples compared to dry specimens. The temperature-scan tests revealed that the hydration condition, but not DC%, significantly affected the behavior of tan δ. However, E′ was not strongly influenced either by DC% or by water content. These results suggest that at a constant frequency the denaturation of collagen triple-helical molecules may have few effects on the viscoelasticity of bone, but moisture may play a prominent role in determining this property.
AB - The purpose of this study was to explore the effects of changes in Type I collagen on the viscoelasticity of bone. Bone coupons were heated at either 100 or 200 °C to induce the thermal denaturation of Type I collagen. Half of these specimens were rehydrated after heat treatment; the other half were tested in a dry condition. The degree of denatured collagen (DC%) was analyzed by a selective digestion technique with the use of α-chymotrypsin. Isothermal (37 °C) and variable temperature tests (scans from 35 to 200 °C) were performed with the use of a dynamic mechanical analyzer to evaluate changes in bone viscoelastic properties as a function of collagen damage, specifically, changes in the loss factor (tan δ) and storage modulus (E′) were assessed. Significant collagen denaturation occurred only when bone was heated at 200 °C irrespective of the hydration condition. Also, DC% did not show a significant effect on tan δ. However, higher values of tan δ were observed in wet samples compared to dry specimens. The temperature-scan tests revealed that the hydration condition, but not DC%, significantly affected the behavior of tan δ. However, E′ was not strongly influenced either by DC% or by water content. These results suggest that at a constant frequency the denaturation of collagen triple-helical molecules may have few effects on the viscoelasticity of bone, but moisture may play a prominent role in determining this property.
KW - Collagen denaturation
KW - Cortical bone
KW - Loss tangent
KW - Moisture
KW - Viscoelasticity
UR - http://www.scopus.com/inward/record.url?scp=0036151497&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036151497&partnerID=8YFLogxK
U2 - 10.1002/jbm.10086
DO - 10.1002/jbm.10086
M3 - Article
C2 - 11787026
AN - SCOPUS:0036151497
VL - 63
SP - 31
EP - 36
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 0021-9304
IS - 1
ER -