TY - JOUR
T1 - Coelomycetous fungi in the clinical setting
T2 - Morphological convergence and cryptic diversity
AU - Valenzuela-Lopez, Nicomedes
AU - Sutton, Deanna A.
AU - Cano-Lira, José F.
AU - Paredes, Katihuska
AU - Wiederhold, Nathan
AU - Guarro, Josep
AU - Stchigel, Alberto M.
N1 - Publisher Copyright:
© 2017 American Society for Microbiology.
PY - 2017/2
Y1 - 2017/2
N2 - Human infections by coelomycetous fungi are becoming more frequent and range from superficial to systemic dissemination. Traumatic implantation of contaminated plant material is the most common cause. The typical morphological feature of these fungi is the production of asexual spores (conidia) within fruiting bodies called conidiomata. This study aimed to determine the distribution of the coelomycetes in clinical samples by a phenotypic and molecular study of a large set of isolates received from a U.S. Reference mycological institution and by obtaining the in vitro antifungal susceptibility pattern of nine antifungals against a selected group of isolates. A total of 230 isolates were identified by sequencing the D1 and D2 domains of the large subunit (LSU) nuclear ribosomal RNA (nrRNA) gene and by morphological characterization. Eleven orders of the phylum Ascomycota were identified: Pleosporales (the largest group; 66.1%), Botryosphaeriales (19.57%), Glomerellales (4.35%), Diaporthales (3.48%), Xylariales (2.17%), Hysteriales and Valsariales (0.87%), and Capnodiales, Helotiales, Hypocreales and Magnaporthales (0.43% each). The most prevalent species were Neoscytalidium dimidiatum, Paraconiothyrium spp., Phoma herbarum, Didymella heteroderae, and Epicoccum sorghinum. The most common anatomical site of isolation was superficial tissue (66.5%), followed by the respiratory tract (17.4%). Most of the isolates tested were susceptible to the majority of antifungals, and only flucytosine showed poor antifungal activity.
AB - Human infections by coelomycetous fungi are becoming more frequent and range from superficial to systemic dissemination. Traumatic implantation of contaminated plant material is the most common cause. The typical morphological feature of these fungi is the production of asexual spores (conidia) within fruiting bodies called conidiomata. This study aimed to determine the distribution of the coelomycetes in clinical samples by a phenotypic and molecular study of a large set of isolates received from a U.S. Reference mycological institution and by obtaining the in vitro antifungal susceptibility pattern of nine antifungals against a selected group of isolates. A total of 230 isolates were identified by sequencing the D1 and D2 domains of the large subunit (LSU) nuclear ribosomal RNA (nrRNA) gene and by morphological characterization. Eleven orders of the phylum Ascomycota were identified: Pleosporales (the largest group; 66.1%), Botryosphaeriales (19.57%), Glomerellales (4.35%), Diaporthales (3.48%), Xylariales (2.17%), Hysteriales and Valsariales (0.87%), and Capnodiales, Helotiales, Hypocreales and Magnaporthales (0.43% each). The most prevalent species were Neoscytalidium dimidiatum, Paraconiothyrium spp., Phoma herbarum, Didymella heteroderae, and Epicoccum sorghinum. The most common anatomical site of isolation was superficial tissue (66.5%), followed by the respiratory tract (17.4%). Most of the isolates tested were susceptible to the majority of antifungals, and only flucytosine showed poor antifungal activity.
KW - Antifungal susceptibility
KW - Coelomycetes
KW - Coelomycetous fungi
KW - Colletotrichum
KW - Mycosis
KW - Neoscytalidium
KW - Phoma
KW - Pyrenochaeta
UR - http://www.scopus.com/inward/record.url?scp=85012016822&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85012016822&partnerID=8YFLogxK
U2 - 10.1128/JCM.02221-16
DO - 10.1128/JCM.02221-16
M3 - Article
C2 - 27927918
AN - SCOPUS:85012016822
SN - 0095-1137
VL - 55
SP - 552
EP - 567
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 2
ER -