Coelogin ameliorates metabolic dyshomeostasis by regulating adipogenesis and enhancing energy expenditure in adipose tissue

Sanchita Gupta, Tripti Mishra, Salil Varshney, Vinita Kushawaha, Nilesh Khandelwal, Prashant Rai, Richa Garg, Kapil Dev, Abhishek Gupta, Durgesh Kumar, Vishal M. Balaramnavar, Kamal Ram Arya, Jiaur R. Gayen, Tadigoppula Narender, Anil Nilkanth Gaikwad

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Obesity and associated metabolic disorders are heading up with an alarming rate in developing nations. One of highly sought solution for metabolic disorders is to identify natural molecule with an ability to reduce obesity and increase insulin sensitivity. Coelogin (CLN) is a phenanthrene derivative isolated from the ethanolic extract of Coelogyne cristata. In our constant efforts to identify novel anti-dyslipidemic and anti-adipogenic compounds using CFPMA (common feature pharmacophore model using known anti-adipogenic compounds) model, predicted possible anti-adipogenic activity of CLN. In vitro results showed significant inhibition of adipogenesis in 3T3-L1 and C3H10T1/2 cell by CLN. It arrests the cell cycle in G1 phase of interphase and inhibits mitotic clonal expansion to regulate adipogenesis. CLN elicits insulin sensitizing effect in mature adipocytes. During extracellular flux assessment studies, it increases oxidative respiration and energy expenditure in adipocytes. In vivo, CLN reversed HFD-induced dyslipidemia as well as insulin resistance in C57BL/6 mice. It promoted the expression of genes involved in improved mitochondrial function and fatty acid oxidation in eWAT. CLN restored energy expenditure and increased the capacity of energy utilization in HFD fed mice. Taken together, the study indicated beneficial effects of CLN in combating obesity-associated metabolic complications.

Original languageEnglish (US)
Article number105776
JournalPharmacological Research
Volume172
DOIs
StatePublished - Oct 2021
Externally publishedYes

Keywords

  • Adipogenesis
  • Adipose tissue
  • Anti-adipogenic
  • Anti-dyslipidemic
  • Coelogin
  • Coelogyne cristata
  • Energy expenditure
  • HFD
  • Insulin Resistance
  • Obesity
  • Phenanthrene

ASJC Scopus subject areas

  • Pharmacology

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