Abstract
In HEK 293 cells expressing the human dopamine transporter (DAT), a 10-min incubation with 10 μM cocaine followed by extensive washing resulted in a 30% increase in [3H]dopamine (DA) uptake as well as an increase in cell surface DAT in biotinylation experiments. Consistent with this novel regulation, [3H]DA uptake into synaptosomes prepared from the nucleus accumbens of rats sacrificed 30 min after a single cocaine injection (30 mg/kg) was significantly increased compared to controls (56% increase in Vmax, no change in Km). In addition, DA clearance in the striatum of anesthetized rats was increased after local application of a low (3 pmol) but not high (65 pmol) dose of cocaine, presumably as a result of mobilization of DAT to the cell surface. Cocaine-induced increases in cell surface expression of DAT and associated changes in DA clearance represent a novel mechanism that may play a role in its addictive properties.
Original language | English (US) |
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Pages (from-to) | 1545-1550 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 290 |
Issue number | 5 |
DOIs | |
State | Published - 2002 |
Keywords
- Biotinylation
- Chronoamperometry
- Cocaine
- Dopamine transporter
- Nucleus accumbens
- Striatum
- Trafficking
- Uptake
ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
- Cell Biology