Co-Expression of Urokinase With Haptoglobin in Human Carcinomas

Shashikumar Harvey, Shin Kohga, Sheila N.J. Sait, Gabor Markus, Thelma C. Hurd, Maisie Martinick, Joseph Geradts, Rakhee Saxena, John F. Gibbs

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Background: We have shown that colon and breast cancer contains large amounts of urokinase (uPA), and that these cells are the actual sites of its synthesis. We isolated a large complex molecule consisting of the β-chain of uPA, both chains of haptoglobin (Hp), and part or all of an NCAM-like molecule. The question arose whether it would be possible to show the presence of Hp in the same cells where uPA was found. Materials and methods: Human colon and breast adenocarcinomas were investigated for expression of Hp and uPA by immunohistochemistry. Fluorescence in situ hybridization was used to identify the cells of origin of these antigens. Results: Hp was expressed in 8 of 11 colon adenocarcinomas, and in 10 of 12 breast tumors. uPA was demonstrable on the cell membrane and in the cytoplasm of all 11 colon adenocarcinomas studied, and cytoplasmic uPA-mRNA was found in all cases. While uPA was also detected in some stromal and inflammatory cells, Hp was present abundantly in such cells, as well as in capillary endothelial cells. Hp-mRNA was also found in both colon and breast tumors wherever the antigens were expressed. Conclusions: uPA and Hp are produced by the cancer cells and are not taken up by stromal elements. While the role of uPA in the malignant process is well documented, that of Hp is largely unexplored. Its ubiquity, shown here, suggests that it is also involved in some aspects of that process.

Original languageEnglish (US)
Pages (from-to)189-197
Number of pages9
JournalJournal of Surgical Research
Volume152
Issue number2
DOIs
StatePublished - Apr 1 2009

Keywords

  • FISH
  • haptoglobin
  • immunohistochemistry
  • tumor markers
  • urokinase

ASJC Scopus subject areas

  • Surgery

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    Harvey, S., Kohga, S., Sait, S. N. J., Markus, G., Hurd, T. C., Martinick, M., Geradts, J., Saxena, R., & Gibbs, J. F. (2009). Co-Expression of Urokinase With Haptoglobin in Human Carcinomas. Journal of Surgical Research, 152(2), 189-197. https://doi.org/10.1016/j.jss.2008.06.016