Cl- transport in basolateral renal medullary vesicles: I. Cl- transport in intact vesicles

John M. Bayliss, W. Brian Reeves, Thomas E. Andreoli

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

This paper provides the results of studies which characterized conductive36Cl- flux in basolaterally enriched membrane vesicles prepared from rabbit renal outer medulla. Conductive36Cl- uptake was studied under two different experimental conditions. In the first,36Cl- flux was driven by an inside positive voltage created with oppositely directed Cl- and gluconate gradients. In the second, an inwardly direct K+ gradient was used to drive36Cl- uptake. By these two methods, voltage-sensitive36Cl- uptake was shown to comprise about 45 and 65%, respectively, of the initial rates of total36Cl- flux. Separate paired studies demonstrated that the conductive36Cl- uptake was inhibited by the Cl- channel blocker diphenylamine-2-carboxylate (DPC) with an IC50 for DPC of 154 μm. The voltagedependent36Cl- uptake had an activation energy of 6.4 kcal/mole. This36Cl- conductance had an anion selectivity sequence of I->Cl-≧NO3-≫gluconate.

Original languageEnglish (US)
Pages (from-to)49-56
Number of pages8
JournalThe Journal of Membrane Biology
Volume113
Issue number1
DOIs
StatePublished - Jan 1 1990

    Fingerprint

Keywords

  • Cl channels/bilayers
  • Cl channels/vesicles
  • channel conductance
  • rectification
  • thick ascending limb

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

Cite this