Cl^- transport in an immortalized human epithelial cell line (NCM460) derived from the normal transverse colon

Cells of a newly described, immortalized, epithelial, human transverse colonic cell line, NCM460, reach ~90% confluence on plastic and develop transepithelial resistances of 120-250 Ω·cm² on porous substrates. Its utility as a model for the transverse human colon was validated by comparing second messenger-mediated Cl^- transport, using the fluorescent probe 6- methoxy-quinolyl acetoethyl ester, in NCM460 cells and colonocytes isolated from human transverse crypts. Basal Cl^- influx was increased (P < 0.01) by PGE₁ (1 μM), forskolin (1 μM), 8-bromoadenosine 3'5'-cyclic monophosphate (100 μM), heat-stable Escherichia coli enterotoxin (STa; 1 μM), 8- bromoguanosine 3'5'-cyclic monophosphate (100 μM), histamine (1 μM), and phorbol 12,13-dibutyrate (1 μM) in both cell types. The Cl^- channel blocker diphenylamine 2-carboxylic acid (50 μM) and the Na⁺-K⁺-2Cl^- cotransport inhibitor furosemide (1 μM), but not the K⁺ channel blocker Ba²⁺ (3 mM), inhibited these Cl^- permeabilities. These cells possess transcripts for cystic fibrosis transmembrane conductance regulator, Na⁺-K⁺-2Cl^- cotransporter, STa receptor, and intestine-specific cGMP-dependent protein kinase II. Thus cAMP-, cGMP-, and Ca²⁺-dependent secretagogues act on NCM460 and primary colonocytes to stimulate Cl^- transport. This validates the utility of NCM460 as a model for transverse colonic crypts and is the first demonstration of a colonic cell line whose origin is known.