Cl- channels in basolateral renal medullary vesicles X. Cloning of a Cl- channel from rabbit outer medulla

Ludwika Zimniak; Christopher J. Winters; W. Brian Reeves; Thomas E. Andreoli

doi:10.1038/ki.1995.481

Cl^- channels in basolateral renal medullary vesicles X. Cloning of a Cl^- channel from rabbit outer medulla

Ludwika Zimniak, Christopher J. Winters, W. Brian Reeves, Thomas E. Andreoli

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

These experiments were intended to identify candidate cDNAs which might encode basolateral membrane Cl^- channels of the mTAL using a homology-based cloning strategy. We prepared a cDNA library using a 1.8 to 3.2 kb mRNA fraction from rabbit outer medulla that induces a Cl^- conductance in cellular membranes of Xenopus/laevis oocytes. The cDNA library was screened with two ³²P-oligonucleotide probes corresponding to highly conserved sequences in other Cl^- channels. We isolated two cDNAs: rbClC-Ka and rbC/C-Kb. The protein sequences deduced from these two cDNAs had 99% homology. Using RT-PCR technology, cultured mouse mTAL cells were found to contain mRNA corresponding to these two cDNAs. Expression of the mRNAs corresponding to these two cDNAs was kidney-specific and was greater in rabbit renal medulla than rabbit renal cortex. Finally, by using RT-PCR technology in combination with microdissected glomeruli or tubule segments, we found mRNA for rbClC-Ka in glomeruli, proximal convoluted tubules, mTAL and cortical collecting tubules.

Original language	English (US)
Pages (from-to)	1828-1836
Number of pages	9
Journal	Kidney international
Volume	48
Issue number	6
DOIs	https://doi.org/10.1038/ki.1995.481
State	Published - Dec 1995
Externally published	Yes

ASJC Scopus subject areas

Nephrology

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@article{dab6901fb8e34b6da822d7e7b56fb10e,

title = "Cl- channels in basolateral renal medullary vesicles X. Cloning of a Cl- channel from rabbit outer medulla",

abstract = "These experiments were intended to identify candidate cDNAs which might encode basolateral membrane Cl- channels of the mTAL using a homology-based cloning strategy. We prepared a cDNA library using a 1.8 to 3.2 kb mRNA fraction from rabbit outer medulla that induces a Cl- conductance in cellular membranes of Xenopus/laevis oocytes. The cDNA library was screened with two 32P-oligonucleotide probes corresponding to highly conserved sequences in other Cl- channels. We isolated two cDNAs: rbClC-Ka and rbC/C-Kb. The protein sequences deduced from these two cDNAs had 99% homology. Using RT-PCR technology, cultured mouse mTAL cells were found to contain mRNA corresponding to these two cDNAs. Expression of the mRNAs corresponding to these two cDNAs was kidney-specific and was greater in rabbit renal medulla than rabbit renal cortex. Finally, by using RT-PCR technology in combination with microdissected glomeruli or tubule segments, we found mRNA for rbClC-Ka in glomeruli, proximal convoluted tubules, mTAL and cortical collecting tubules.",

author = "Ludwika Zimniak and Winters, {Christopher J.} and Reeves, {W. Brian} and Andreoli, {Thomas E.}",

note = "Funding Information: This work was supported by NIH Grant (5 ROl DK25540) and a Veterans Administration Merit Review Grant to T. E. Andreoli. W. B.",

year = "1995",

month = dec,

doi = "10.1038/ki.1995.481",

language = "English (US)",

volume = "48",

pages = "1828--1836",

journal = "Kidney international",

issn = "0085-2538",

publisher = "Elsevier B.V.",

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T1 - Cl- channels in basolateral renal medullary vesicles X. Cloning of a Cl- channel from rabbit outer medulla

AU - Zimniak, Ludwika

AU - Winters, Christopher J.

AU - Reeves, W. Brian

AU - Andreoli, Thomas E.

N1 - Funding Information: This work was supported by NIH Grant (5 ROl DK25540) and a Veterans Administration Merit Review Grant to T. E. Andreoli. W. B.

PY - 1995/12

Y1 - 1995/12

N2 - These experiments were intended to identify candidate cDNAs which might encode basolateral membrane Cl- channels of the mTAL using a homology-based cloning strategy. We prepared a cDNA library using a 1.8 to 3.2 kb mRNA fraction from rabbit outer medulla that induces a Cl- conductance in cellular membranes of Xenopus/laevis oocytes. The cDNA library was screened with two 32P-oligonucleotide probes corresponding to highly conserved sequences in other Cl- channels. We isolated two cDNAs: rbClC-Ka and rbC/C-Kb. The protein sequences deduced from these two cDNAs had 99% homology. Using RT-PCR technology, cultured mouse mTAL cells were found to contain mRNA corresponding to these two cDNAs. Expression of the mRNAs corresponding to these two cDNAs was kidney-specific and was greater in rabbit renal medulla than rabbit renal cortex. Finally, by using RT-PCR technology in combination with microdissected glomeruli or tubule segments, we found mRNA for rbClC-Ka in glomeruli, proximal convoluted tubules, mTAL and cortical collecting tubules.

AB - These experiments were intended to identify candidate cDNAs which might encode basolateral membrane Cl- channels of the mTAL using a homology-based cloning strategy. We prepared a cDNA library using a 1.8 to 3.2 kb mRNA fraction from rabbit outer medulla that induces a Cl- conductance in cellular membranes of Xenopus/laevis oocytes. The cDNA library was screened with two 32P-oligonucleotide probes corresponding to highly conserved sequences in other Cl- channels. We isolated two cDNAs: rbClC-Ka and rbC/C-Kb. The protein sequences deduced from these two cDNAs had 99% homology. Using RT-PCR technology, cultured mouse mTAL cells were found to contain mRNA corresponding to these two cDNAs. Expression of the mRNAs corresponding to these two cDNAs was kidney-specific and was greater in rabbit renal medulla than rabbit renal cortex. Finally, by using RT-PCR technology in combination with microdissected glomeruli or tubule segments, we found mRNA for rbClC-Ka in glomeruli, proximal convoluted tubules, mTAL and cortical collecting tubules.

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DO - 10.1038/ki.1995.481

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JO - Kidney international

JF - Kidney international

IS - 6

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Cl^- channels in basolateral renal medullary vesicles X. Cloning of a Cl^- channel from rabbit outer medulla

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