Clonotypic analysis of anti-acetylcholine receptor antibodies produced against native and denatured antigen

Trai Ming Yeh, Keith A Krolick

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Rats were immunized with either conformationally intact acetylcholine receptor (AChR) or reduced and sodium dodecyl sulfate (SDS)-denatured AChR. As expected, challenge with native AChR (nAChR) resulted in the production of serum antibodies reactive with native AChR; these antibodies were, as observed in earlier studies, oligoclonal, dominated by the rat IgG2a subclass, heterogeneous with respect to binding avidity, and importantly, able to interfere with normal AChR-dependent muscle contraction. Antibodies produced as a result of immunization with denatured AChR (dAChR) that were crossreactive with nAChR were similar but not identical to those produced directly against nAChR; in contrast, however, dAChR-stimulated antibodies were clearly incapable of causing detectable impairment of AChR-dependent muscle contractile function. This difference in disease-causing potential was demonstrated in spite of the observation that either nAChR or dAChR could generate similar levels of circulating antibodies that reacted with the native form of AChR. Moreover, the same difference in disease-causing potential was again observed upon passive intravenous administration of anti-AChR antibodies into naive recipient rats. IEF analyses demonstrated that antibodies stimulated by dAChR immunization expressed the same clonotypic heterogeneity and isotype distribution as those stimulated by nAChR immunization. However, an apparent shift was observed in the preferred clonotypes expressed in rats immunized with dAChR; antibodies with relatively lower binding avidities were more markedly represented than the higher avidity antibodies commonly accompanying immunization with nAChR. Furthermore, a distinct subset of high avidity clonotypes was expressed following immunization with dAChR not associated with nAChR immunization.

Original languageEnglish (US)
Pages (from-to)133-142
Number of pages10
JournalJournal of Neuroimmunology
Volume24
Issue number1-2
DOIs
StatePublished - 1989

Fingerprint

Cholinergic Receptors
Antigens
Antibodies
Immunization
Antibody Affinity
Muscle Contraction
Sodium Dodecyl Sulfate
Intravenous Administration
Antibody Formation
Muscles
Serum

Keywords

  • Acetylcholine receptor
  • Isoelectric focusing
  • Myasthenia gravis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

Clonotypic analysis of anti-acetylcholine receptor antibodies produced against native and denatured antigen. / Yeh, Trai Ming; Krolick, Keith A.

In: Journal of Neuroimmunology, Vol. 24, No. 1-2, 1989, p. 133-142.

Research output: Contribution to journalArticle

@article{51fce161f6f14961a1d472d4f434ea40,
title = "Clonotypic analysis of anti-acetylcholine receptor antibodies produced against native and denatured antigen",
abstract = "Rats were immunized with either conformationally intact acetylcholine receptor (AChR) or reduced and sodium dodecyl sulfate (SDS)-denatured AChR. As expected, challenge with native AChR (nAChR) resulted in the production of serum antibodies reactive with native AChR; these antibodies were, as observed in earlier studies, oligoclonal, dominated by the rat IgG2a subclass, heterogeneous with respect to binding avidity, and importantly, able to interfere with normal AChR-dependent muscle contraction. Antibodies produced as a result of immunization with denatured AChR (dAChR) that were crossreactive with nAChR were similar but not identical to those produced directly against nAChR; in contrast, however, dAChR-stimulated antibodies were clearly incapable of causing detectable impairment of AChR-dependent muscle contractile function. This difference in disease-causing potential was demonstrated in spite of the observation that either nAChR or dAChR could generate similar levels of circulating antibodies that reacted with the native form of AChR. Moreover, the same difference in disease-causing potential was again observed upon passive intravenous administration of anti-AChR antibodies into naive recipient rats. IEF analyses demonstrated that antibodies stimulated by dAChR immunization expressed the same clonotypic heterogeneity and isotype distribution as those stimulated by nAChR immunization. However, an apparent shift was observed in the preferred clonotypes expressed in rats immunized with dAChR; antibodies with relatively lower binding avidities were more markedly represented than the higher avidity antibodies commonly accompanying immunization with nAChR. Furthermore, a distinct subset of high avidity clonotypes was expressed following immunization with dAChR not associated with nAChR immunization.",
keywords = "Acetylcholine receptor, Isoelectric focusing, Myasthenia gravis",
author = "Yeh, {Trai Ming} and Krolick, {Keith A}",
year = "1989",
doi = "10.1016/0165-5728(89)90107-0",
language = "English (US)",
volume = "24",
pages = "133--142",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Clonotypic analysis of anti-acetylcholine receptor antibodies produced against native and denatured antigen

AU - Yeh, Trai Ming

AU - Krolick, Keith A

PY - 1989

Y1 - 1989

N2 - Rats were immunized with either conformationally intact acetylcholine receptor (AChR) or reduced and sodium dodecyl sulfate (SDS)-denatured AChR. As expected, challenge with native AChR (nAChR) resulted in the production of serum antibodies reactive with native AChR; these antibodies were, as observed in earlier studies, oligoclonal, dominated by the rat IgG2a subclass, heterogeneous with respect to binding avidity, and importantly, able to interfere with normal AChR-dependent muscle contraction. Antibodies produced as a result of immunization with denatured AChR (dAChR) that were crossreactive with nAChR were similar but not identical to those produced directly against nAChR; in contrast, however, dAChR-stimulated antibodies were clearly incapable of causing detectable impairment of AChR-dependent muscle contractile function. This difference in disease-causing potential was demonstrated in spite of the observation that either nAChR or dAChR could generate similar levels of circulating antibodies that reacted with the native form of AChR. Moreover, the same difference in disease-causing potential was again observed upon passive intravenous administration of anti-AChR antibodies into naive recipient rats. IEF analyses demonstrated that antibodies stimulated by dAChR immunization expressed the same clonotypic heterogeneity and isotype distribution as those stimulated by nAChR immunization. However, an apparent shift was observed in the preferred clonotypes expressed in rats immunized with dAChR; antibodies with relatively lower binding avidities were more markedly represented than the higher avidity antibodies commonly accompanying immunization with nAChR. Furthermore, a distinct subset of high avidity clonotypes was expressed following immunization with dAChR not associated with nAChR immunization.

AB - Rats were immunized with either conformationally intact acetylcholine receptor (AChR) or reduced and sodium dodecyl sulfate (SDS)-denatured AChR. As expected, challenge with native AChR (nAChR) resulted in the production of serum antibodies reactive with native AChR; these antibodies were, as observed in earlier studies, oligoclonal, dominated by the rat IgG2a subclass, heterogeneous with respect to binding avidity, and importantly, able to interfere with normal AChR-dependent muscle contraction. Antibodies produced as a result of immunization with denatured AChR (dAChR) that were crossreactive with nAChR were similar but not identical to those produced directly against nAChR; in contrast, however, dAChR-stimulated antibodies were clearly incapable of causing detectable impairment of AChR-dependent muscle contractile function. This difference in disease-causing potential was demonstrated in spite of the observation that either nAChR or dAChR could generate similar levels of circulating antibodies that reacted with the native form of AChR. Moreover, the same difference in disease-causing potential was again observed upon passive intravenous administration of anti-AChR antibodies into naive recipient rats. IEF analyses demonstrated that antibodies stimulated by dAChR immunization expressed the same clonotypic heterogeneity and isotype distribution as those stimulated by nAChR immunization. However, an apparent shift was observed in the preferred clonotypes expressed in rats immunized with dAChR; antibodies with relatively lower binding avidities were more markedly represented than the higher avidity antibodies commonly accompanying immunization with nAChR. Furthermore, a distinct subset of high avidity clonotypes was expressed following immunization with dAChR not associated with nAChR immunization.

KW - Acetylcholine receptor

KW - Isoelectric focusing

KW - Myasthenia gravis

UR - http://www.scopus.com/inward/record.url?scp=0024460624&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024460624&partnerID=8YFLogxK

U2 - 10.1016/0165-5728(89)90107-0

DO - 10.1016/0165-5728(89)90107-0

M3 - Article

C2 - 2478576

AN - SCOPUS:0024460624

VL - 24

SP - 133

EP - 142

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

IS - 1-2

ER -