Cloning of the proto-oncogene c-src from rat testis

Otor Al-Khalilt; B. J. Duke; Shawn Zeltwanger; Douglas C. Eaton; Bret Spier; James D. Stockand

doi:10.3109/10425170109084469

Cloning of the proto-oncogene c-src from rat testis

Otor Al-Khalilt, B. J. Duke, Shawn Zeltwanger, Douglas C. Eaton, Bret Spier, James D. Stockand

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The cellular homolog of the oncogene v-src, the proto-oncogene c-src, was cloned from rat testis using a high stringency polymerase chain reaction. Rat c-src cDNA shared identity with chicken and mouse, and Rous sarcoma virus c-src and v-src, respectively. Rat c-Src protein was 98% homologous to both human and mouse c-Src. Interestingly, rat Src contained one extra amino acid compared to the mouse protein. As expected, the rat testis Src lacked the six extra residues common to the neuronal Src identified in human and mouse. Reporting of the cDNA sequence for non-neuronal, rat c-src should facilitate experimentation into cell growth and transformation using rat tissues as models of human disease.

Original language	English (US)
Pages (from-to)	425-429
Number of pages	5
Journal	Mitochondrial DNA
Volume	12
Issue number	5-6
DOIs	https://doi.org/10.3109/10425170109084469
State	Published - 2001
Externally published	Yes

Keywords

Phosphoprotein
Proto-oncogene
Src
pp60c-Src

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.3109/10425170109084469

Cite this

@article{df7c240ece99425d99b9ebf4e75fc51c,

title = "Cloning of the proto-oncogene c-src from rat testis",

abstract = "The cellular homolog of the oncogene v-src, the proto-oncogene c-src, was cloned from rat testis using a high stringency polymerase chain reaction. Rat c-src cDNA shared identity with chicken and mouse, and Rous sarcoma virus c-src and v-src, respectively. Rat c-Src protein was 98% homologous to both human and mouse c-Src. Interestingly, rat Src contained one extra amino acid compared to the mouse protein. As expected, the rat testis Src lacked the six extra residues common to the neuronal Src identified in human and mouse. Reporting of the cDNA sequence for non-neuronal, rat c-src should facilitate experimentation into cell growth and transformation using rat tissues as models of human disease.",

keywords = "Phosphoprotein, Proto-oncogene, Src, pp60c-Src",

author = "Otor Al-Khalilt and Duke, {B. J.} and Shawn Zeltwanger and Eaton, {Douglas C.} and Bret Spier and Stockand, {James D.}",

year = "2001",

doi = "10.3109/10425170109084469",

language = "English (US)",

volume = "12",

pages = "425--429",

journal = "Mitochondrial DNA",

issn = "1940-1736",

publisher = "Informa Healthcare",

number = "5-6",

}

TY - JOUR

T1 - Cloning of the proto-oncogene c-src from rat testis

AU - Al-Khalilt, Otor

AU - Duke, B. J.

AU - Zeltwanger, Shawn

AU - Eaton, Douglas C.

AU - Spier, Bret

AU - Stockand, James D.

PY - 2001

Y1 - 2001

N2 - The cellular homolog of the oncogene v-src, the proto-oncogene c-src, was cloned from rat testis using a high stringency polymerase chain reaction. Rat c-src cDNA shared identity with chicken and mouse, and Rous sarcoma virus c-src and v-src, respectively. Rat c-Src protein was 98% homologous to both human and mouse c-Src. Interestingly, rat Src contained one extra amino acid compared to the mouse protein. As expected, the rat testis Src lacked the six extra residues common to the neuronal Src identified in human and mouse. Reporting of the cDNA sequence for non-neuronal, rat c-src should facilitate experimentation into cell growth and transformation using rat tissues as models of human disease.

AB - The cellular homolog of the oncogene v-src, the proto-oncogene c-src, was cloned from rat testis using a high stringency polymerase chain reaction. Rat c-src cDNA shared identity with chicken and mouse, and Rous sarcoma virus c-src and v-src, respectively. Rat c-Src protein was 98% homologous to both human and mouse c-Src. Interestingly, rat Src contained one extra amino acid compared to the mouse protein. As expected, the rat testis Src lacked the six extra residues common to the neuronal Src identified in human and mouse. Reporting of the cDNA sequence for non-neuronal, rat c-src should facilitate experimentation into cell growth and transformation using rat tissues as models of human disease.

KW - Phosphoprotein

KW - Proto-oncogene

KW - Src

KW - pp60c-Src

UR - http://www.scopus.com/inward/record.url?scp=0035701151&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035701151&partnerID=8YFLogxK

U2 - 10.3109/10425170109084469

DO - 10.3109/10425170109084469

M3 - Article

C2 - 11913791

AN - SCOPUS:0035701151

SN - 1940-1736

VL - 12

SP - 425

EP - 429

JO - Mitochondrial DNA

JF - Mitochondrial DNA

IS - 5-6

ER -

Cloning of the proto-oncogene c-src from rat testis

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this