Cloning of the proto-oncogene c-src from rat testis

Otor Al-Khalilt, B. J. Duke, Shawn Zeltwanger, Douglas C. Eaton, Bret Spier, James D Stockand

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The cellular homolog of the oncogene v-src, the proto-oncogene c-src, was cloned from rat testis using a high stringency polymerase chain reaction. Rat c-src cDNA shared identity with chicken and mouse, and Rous sarcoma virus c-src and v-src, respectively. Rat c-Src protein was 98% homologous to both human and mouse c-Src. Interestingly, rat Src contained one extra amino acid compared to the mouse protein. As expected, the rat testis Src lacked the six extra residues common to the neuronal Src identified in human and mouse. Reporting of the cDNA sequence for non-neuronal, rat c-src should facilitate experimentation into cell growth and transformation using rat tissues as models of human disease.

Original languageEnglish (US)
Pages (from-to)425-429
Number of pages5
JournalMitochondrial DNA
Volume12
Issue number5-6
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

src Genes
Cloning
Testis
Rats
Organism Cloning
Complementary DNA
Murine Sarcoma Viruses
Rous sarcoma virus
Polymerase chain reaction
Cell growth
Viruses
Oncogenes
Chickens
Proteins
Tissue
Amino Acids
Polymerase Chain Reaction
Growth

Keywords

  • Phosphoprotein
  • pp60c-Src
  • Proto-oncogene
  • Src

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry
  • Endocrinology

Cite this

Al-Khalilt, O., Duke, B. J., Zeltwanger, S., Eaton, D. C., Spier, B., & Stockand, J. D. (2001). Cloning of the proto-oncogene c-src from rat testis. Mitochondrial DNA, 12(5-6), 425-429. https://doi.org/10.3109/10425170109084469

Cloning of the proto-oncogene c-src from rat testis. / Al-Khalilt, Otor; Duke, B. J.; Zeltwanger, Shawn; Eaton, Douglas C.; Spier, Bret; Stockand, James D.

In: Mitochondrial DNA, Vol. 12, No. 5-6, 2001, p. 425-429.

Research output: Contribution to journalArticle

Al-Khalilt, O, Duke, BJ, Zeltwanger, S, Eaton, DC, Spier, B & Stockand, JD 2001, 'Cloning of the proto-oncogene c-src from rat testis', Mitochondrial DNA, vol. 12, no. 5-6, pp. 425-429. https://doi.org/10.3109/10425170109084469
Al-Khalilt O, Duke BJ, Zeltwanger S, Eaton DC, Spier B, Stockand JD. Cloning of the proto-oncogene c-src from rat testis. Mitochondrial DNA. 2001;12(5-6):425-429. https://doi.org/10.3109/10425170109084469
Al-Khalilt, Otor ; Duke, B. J. ; Zeltwanger, Shawn ; Eaton, Douglas C. ; Spier, Bret ; Stockand, James D. / Cloning of the proto-oncogene c-src from rat testis. In: Mitochondrial DNA. 2001 ; Vol. 12, No. 5-6. pp. 425-429.
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