Cloning of the nucleic acid-binding domain of the rat HnRNP C-type protein

Z. Dave Sharp; Kelly P. Smith; Zhaodan Cao; Sharon Helsel

doi:10.1016/0167-4781(90)90073-B

Cloning of the nucleic acid-binding domain of the rat HnRNP C-type protein

Z. Dave Sharp, Kelly P. Smith, Zhaodan Cao, Sharon Helsel

Molecular Medicine

Research output: Contribution to journal › Article

8 Scopus citations

Abstract

A cDNA encoding the nucleic acid-binding domain of the hnRNP C-type protein has been cloned by DNA-affinity screening of pituitary-derived expression libraries. An analysis revealed sequence identity with the human C-type cDNA and demonstrated the presence of a peptide sequence contained within the single-stranded DNA-binding protein, UP2, which was absent from the human cDNA. Structural analysis of the protein encoded by the rat cDNA demonstrated a net charge of + 15 with 14.56% and 6.33% lysines and arginines, respectively, and an amino acid sequence that is consistent with an extensive helix-loop-helix-turn-helix structure.

Original language	English (US)
Pages (from-to)	306-309
Number of pages	4
Journal	BBA - Gene Structure and Expression
Volume	1048
Issue number	2-3
DOIs	https://doi.org/10.1016/0167-4781(90)90073-B
Publication status	Published - Apr 6 1990

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Keywords

(Rat)
C protein
DNA-binding
Helix-loop-helix
Helix-turn-helix
HnRNP

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

Cite this

Sharp, Z. D., Smith, K. P., Cao, Z., & Helsel, S. (1990). Cloning of the nucleic acid-binding domain of the rat HnRNP C-type protein. BBA - Gene Structure and Expression, 1048(2-3), 306-309. https://doi.org/10.1016/0167-4781(90)90073-B

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Access to Document

10.1016/0167-4781(90)90073-B