Cloning of the nucleic acid-binding domain of the rat HnRNP C-type protein

Z. Dave Sharp, Kelly P. Smith, Zhaodan Cao, Sharon Helsel

Research output: Contribution to journalArticle

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Abstract

A cDNA encoding the nucleic acid-binding domain of the hnRNP C-type protein has been cloned by DNA-affinity screening of pituitary-derived expression libraries. An analysis revealed sequence identity with the human C-type cDNA and demonstrated the presence of a peptide sequence contained within the single-stranded DNA-binding protein, UP2, which was absent from the human cDNA. Structural analysis of the protein encoded by the rat cDNA demonstrated a net charge of + 15 with 14.56% and 6.33% lysines and arginines, respectively, and an amino acid sequence that is consistent with an extensive helix-loop-helix-turn-helix structure.

Original languageEnglish (US)
Pages (from-to)306-309
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1048
Issue number2-3
DOIs
Publication statusPublished - Apr 6 1990

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Keywords

  • (Rat)
  • C protein
  • DNA-binding
  • Helix-loop-helix
  • Helix-turn-helix
  • HnRNP

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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