Cloning of the 5′-flanking region of the murine bone morphogenetic protein-7 gene

Matthias Simon, Denis Feliers, Mazen Y Arar, Basant Bhandari, Hanna E. Abboud

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

BMP-7, a member of the bone morphogenetic protein subfamily of the TGFβ-superfamily is highly expressed in the murine kidney. BMP-7 is involved in fetal nephron development and mesenchymal to epithelial cell differentiation. Constitutive BMP-7 expression is found in tubular and glomerular epithelial cells of the adult kidney. BMP-7 may play a role in physiology and pathophysiology of the adult kidney since BMP-7 gene expression in acute renal ischemia is diminished and injection of recombinant BMP-7 into rats with ischemic acute renal failure preserves renal function. In order to investigate the transcriptional regulation of BMP-7, this study was undertaken to clone and characterize the promoter of the murine BMP-7 gene. A 1394 bp sequence of the 5′-flanking region of the BMP-7 gene was isolated and subcloned. No TATA and CAAT box consensus motifs could be identified as shown for promoters of other BMPs. Using in vitro transfection assays, the 5′-flanking region revealed moderate to strong basal promoter activity. PMA increased basal BMP-7 promoter activity. Thus BMP-7 gene transcription might involve at least in part a PKC-dependent pathway. The cloning of a 5′-flanking region of the BMP-7 gene should provide a useful tool for future studies on the transcriptional regulation of BMP-7 gene expression.

Original languageEnglish (US)
Pages (from-to)31-37
Number of pages7
JournalMolecular and Cellular Biochemistry
Volume233
Issue number1-2
DOIs
StatePublished - 2002

Fingerprint

Bone Morphogenetic Protein 7
5' Flanking Region
Cloning
Organism Cloning
Genes
Kidney
Gene expression
Epithelial Cells
Gene Expression
TATA Box
Bone Morphogenetic Proteins
Nephrons
Physiology
Transcription
Fetal Development
Acute Kidney Injury

Keywords

  • BMP-7
  • Bone morphogenetic protein
  • Kidney development
  • Promoter activity
  • Transcriptional regulation

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Cloning of the 5′-flanking region of the murine bone morphogenetic protein-7 gene. / Simon, Matthias; Feliers, Denis; Arar, Mazen Y; Bhandari, Basant; Abboud, Hanna E.

In: Molecular and Cellular Biochemistry, Vol. 233, No. 1-2, 2002, p. 31-37.

Research output: Contribution to journalArticle

Simon, Matthias ; Feliers, Denis ; Arar, Mazen Y ; Bhandari, Basant ; Abboud, Hanna E. / Cloning of the 5′-flanking region of the murine bone morphogenetic protein-7 gene. In: Molecular and Cellular Biochemistry. 2002 ; Vol. 233, No. 1-2. pp. 31-37.
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