Cloning of SmNCoA-62, a novel nuclear receptor co-activator from Schistosoma mansoni

Assembly of a complex with a SmRXR1/SmNR1 heterodimer, SmGCN5 and SmCBP1

Marcelo Rosado Fantappié, Francisco Meirelles Bastos de Oliveira, Renata de Moraes Maciel, Franklin David Rumjanek, Wenjie Wu, Philip T Loverde

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The Schistosoma mansoni nuclear receptors (NR) SmRXR1 and SmNR1 have recently been shown to form a heterodimer and to bind to canonic hormone response DNA elements. Recruitment of co-regulatory proteins to NRs is required for their transcriptional and biological activities. Here, we cloned a novel S. mansoni NR co-activator, SmNCoA-62. SmNCoA-62 is highly homologous to the human Vitamin D receptor co-activator NCoA62/SKIP. SmNCoA-62 contains the SNW nuclear receptor interaction domain and a putative C-terminus transactivation domain. By using in vitro pull-down assays, we fully mapped the interaction domains of S. mansoni NR co-activators, SmNCoA-62, SmGCN5 and SmCBP1 with SmRXR1 and SmNR1, as well as the domains that mediate interactions amongst the co-activators themselves. By mutagenesis analysis, we showed that SmCBP1 LxxLL motif 2 and LxxLL motif 3, but not LxxLL motif 1, were essential to mediate the interactions of SmCBP1 with the EF domains of SmRXR1 and SmNR1. Histone acetyltransferases SmGCN5 and SmCBP1 specifically acetylated the C/D domains of SmRXR1 and SmNR1. In addition, two acetylation sites of SmNR1 were identified. SmGCN5 and SmCBP1 also acetylated SmNCoA-62 but with significant differences in their acetylation activities. Using gel shift analysis, we were able to demonstrate, in vitro, the assembly of the co-activators on the SmRXR1/SmNR1 heterodimer bound to DNA. LxxLL motifs 2 and 3 of SmCBP1 seemed to play a crucial role for the assembly of the co-activators to the DNA-bound SmRXR1/SmNR1 heterodimer.

Original languageEnglish (US)
Pages (from-to)1133-1147
Number of pages15
JournalInternational Journal for Parasitology
Volume38
Issue number10
DOIs
StatePublished - Aug 2008
Externally publishedYes

Fingerprint

Schistosoma mansoni
Cytoplasmic and Nuclear Receptors
Organism Cloning
Acetylation
DNA
Histone Acetyltransferases
Calcitriol Receptors
Response Elements
Electrophoretic Mobility Shift Assay
Mutagenesis
Transcriptional Activation
Hormones
Proteins
In Vitro Techniques

Keywords

  • Co-activator
  • Gene regulation
  • Nuclear receptor
  • Schistosoma mansoni

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Cloning of SmNCoA-62, a novel nuclear receptor co-activator from Schistosoma mansoni : Assembly of a complex with a SmRXR1/SmNR1 heterodimer, SmGCN5 and SmCBP1. / Fantappié, Marcelo Rosado; de Oliveira, Francisco Meirelles Bastos; de Moraes Maciel, Renata; Rumjanek, Franklin David; Wu, Wenjie; Loverde, Philip T.

In: International Journal for Parasitology, Vol. 38, No. 10, 08.2008, p. 1133-1147.

Research output: Contribution to journalArticle

Fantappié, Marcelo Rosado ; de Oliveira, Francisco Meirelles Bastos ; de Moraes Maciel, Renata ; Rumjanek, Franklin David ; Wu, Wenjie ; Loverde, Philip T. / Cloning of SmNCoA-62, a novel nuclear receptor co-activator from Schistosoma mansoni : Assembly of a complex with a SmRXR1/SmNR1 heterodimer, SmGCN5 and SmCBP1. In: International Journal for Parasitology. 2008 ; Vol. 38, No. 10. pp. 1133-1147.
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abstract = "The Schistosoma mansoni nuclear receptors (NR) SmRXR1 and SmNR1 have recently been shown to form a heterodimer and to bind to canonic hormone response DNA elements. Recruitment of co-regulatory proteins to NRs is required for their transcriptional and biological activities. Here, we cloned a novel S. mansoni NR co-activator, SmNCoA-62. SmNCoA-62 is highly homologous to the human Vitamin D receptor co-activator NCoA62/SKIP. SmNCoA-62 contains the SNW nuclear receptor interaction domain and a putative C-terminus transactivation domain. By using in vitro pull-down assays, we fully mapped the interaction domains of S. mansoni NR co-activators, SmNCoA-62, SmGCN5 and SmCBP1 with SmRXR1 and SmNR1, as well as the domains that mediate interactions amongst the co-activators themselves. By mutagenesis analysis, we showed that SmCBP1 LxxLL motif 2 and LxxLL motif 3, but not LxxLL motif 1, were essential to mediate the interactions of SmCBP1 with the EF domains of SmRXR1 and SmNR1. Histone acetyltransferases SmGCN5 and SmCBP1 specifically acetylated the C/D domains of SmRXR1 and SmNR1. In addition, two acetylation sites of SmNR1 were identified. SmGCN5 and SmCBP1 also acetylated SmNCoA-62 but with significant differences in their acetylation activities. Using gel shift analysis, we were able to demonstrate, in vitro, the assembly of the co-activators on the SmRXR1/SmNR1 heterodimer bound to DNA. LxxLL motifs 2 and 3 of SmCBP1 seemed to play a crucial role for the assembly of the co-activators to the DNA-bound SmRXR1/SmNR1 heterodimer.",
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AU - Fantappié, Marcelo Rosado

AU - de Oliveira, Francisco Meirelles Bastos

AU - de Moraes Maciel, Renata

AU - Rumjanek, Franklin David

AU - Wu, Wenjie

AU - Loverde, Philip T

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AB - The Schistosoma mansoni nuclear receptors (NR) SmRXR1 and SmNR1 have recently been shown to form a heterodimer and to bind to canonic hormone response DNA elements. Recruitment of co-regulatory proteins to NRs is required for their transcriptional and biological activities. Here, we cloned a novel S. mansoni NR co-activator, SmNCoA-62. SmNCoA-62 is highly homologous to the human Vitamin D receptor co-activator NCoA62/SKIP. SmNCoA-62 contains the SNW nuclear receptor interaction domain and a putative C-terminus transactivation domain. By using in vitro pull-down assays, we fully mapped the interaction domains of S. mansoni NR co-activators, SmNCoA-62, SmGCN5 and SmCBP1 with SmRXR1 and SmNR1, as well as the domains that mediate interactions amongst the co-activators themselves. By mutagenesis analysis, we showed that SmCBP1 LxxLL motif 2 and LxxLL motif 3, but not LxxLL motif 1, were essential to mediate the interactions of SmCBP1 with the EF domains of SmRXR1 and SmNR1. Histone acetyltransferases SmGCN5 and SmCBP1 specifically acetylated the C/D domains of SmRXR1 and SmNR1. In addition, two acetylation sites of SmNR1 were identified. SmGCN5 and SmCBP1 also acetylated SmNCoA-62 but with significant differences in their acetylation activities. Using gel shift analysis, we were able to demonstrate, in vitro, the assembly of the co-activators on the SmRXR1/SmNR1 heterodimer bound to DNA. LxxLL motifs 2 and 3 of SmCBP1 seemed to play a crucial role for the assembly of the co-activators to the DNA-bound SmRXR1/SmNR1 heterodimer.

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