Cloning of a ε-class glutathione s-transferase complementary DNA and characterization of its glucocorticoid inducibility in a smooth muscle tumor cell line

James S. Norris, David A. Schwartz, Stewart L. MacLeod, Weimin Fan, Timothy J. O'Brien, Stephen E. Harris, Rosario Trifiletti, Laurence E. Cornett, Tina M. Cooper, Wendall M. Levi, Roy G. Smith

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A cDNA (designated hGSTYBX) encompassing the complete coding sequence of a hamster μ-class glutathione S-transferase (GST) subunit was cloned from a λZAP library constructed with mRNA isolated from triamcinolone acetonide-treated smooth muscle tumor cells (DDT1 MF-2). Analysis of its nucleotide and deduced amino acid sequences demonstrated highest homology to the rat μ-class GST YB2 subunit. In proliferating subconfluent cells, in which constitutive expression of hGSTYBX mRNA was undetectable, glucocorticoid treatment induced hGSTYBX expression after a time lag of 3 h, and maximal induction occurred at 10 h. Nuclear run-onanalysis showed that glucocorticoid induction resulted at least in part from an increased rate of transcription. Simultaneous treatment with glucocorticoid and cycloheximide prevented glucocorticoid induction, but had little effect on basal expression in confluent cells. In contrast, cycloheximide treatment 3 h after glucocorticoid treatment resulted in nearly full induction. These results taken together suggest that hGSTYBX induction may be a secondary glucocorticoid response.

Original languageEnglish (US)
Pages (from-to)979-986
Number of pages8
JournalMolecular Endocrinology
Volume5
Issue number7
DOIs
StatePublished - Jul 1 1991

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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