Cloning, expression and genomic structure of human LMX1A, and variant screening in Pima Indians

Farook Thameem, Johanna K. Wolford, Juehu Wang, Michael S. German, Clifton Bogardus, Michal Prochazka

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

LIM-homeodomain containing protein LMX1A activates transcription of the insulin gene. The human LMX1A gene maps to 1q22-q23, a region identified as a putative type 2 diabetes mellitus (T2DM) locus in several different populations. We analyzed LMX1A as a positional and biological candidate gene for T2DM in the Pima Indians, in whom a linkage of T2DM to 1q21-q23 has been previously reported. In the present study, we describe the cloning, expression and genomic organization of the LMX1A gene, which is composed of 11 exons spanning approximately 151 kb. In addition to a transcript encoding the predicted full-length protein of 382 amino acids, we identified two truncated cDNA forms produced via additional transcription start sites and alternative splicing. We identified seven single nucleotide polymorphisms (SNPs) throughout the LMX1A locus and determined allele frequency distributions in 150 diabetic and 150 unaffected Pimas. We did not find evidence for association of any LMX1A SNPs with T2DM and conclude that LMX1A does not contribute significantly to T2DM etiology in Pima Indians.

Original languageEnglish (US)
Pages (from-to)217-225
Number of pages9
JournalGene
Volume290
Issue number1-2
DOIs
StatePublished - May 15 2002
Externally publishedYes

Fingerprint

Potassium Iodide
Type 2 Diabetes Mellitus
Organism Cloning
Genes
Single Nucleotide Polymorphism
LIM-Homeodomain Proteins
Transcription Initiation Site
Alternative Splicing
Gene Frequency
Exons
Complementary DNA
Insulin
Amino Acids
Population
Proteins

Keywords

  • Association analysis
  • Candidate gene
  • Cloning
  • LMX1A
  • Pima Indians
  • Single nucleotide polymorphism
  • Type 2 diabetes mellitus

ASJC Scopus subject areas

  • Genetics

Cite this

Thameem, F., Wolford, J. K., Wang, J., German, M. S., Bogardus, C., & Prochazka, M. (2002). Cloning, expression and genomic structure of human LMX1A, and variant screening in Pima Indians. Gene, 290(1-2), 217-225. https://doi.org/10.1016/S0378-1119(02)00582-6

Cloning, expression and genomic structure of human LMX1A, and variant screening in Pima Indians. / Thameem, Farook; Wolford, Johanna K.; Wang, Juehu; German, Michael S.; Bogardus, Clifton; Prochazka, Michal.

In: Gene, Vol. 290, No. 1-2, 15.05.2002, p. 217-225.

Research output: Contribution to journalArticle

Thameem, F, Wolford, JK, Wang, J, German, MS, Bogardus, C & Prochazka, M 2002, 'Cloning, expression and genomic structure of human LMX1A, and variant screening in Pima Indians', Gene, vol. 290, no. 1-2, pp. 217-225. https://doi.org/10.1016/S0378-1119(02)00582-6
Thameem F, Wolford JK, Wang J, German MS, Bogardus C, Prochazka M. Cloning, expression and genomic structure of human LMX1A, and variant screening in Pima Indians. Gene. 2002 May 15;290(1-2):217-225. https://doi.org/10.1016/S0378-1119(02)00582-6
Thameem, Farook ; Wolford, Johanna K. ; Wang, Juehu ; German, Michael S. ; Bogardus, Clifton ; Prochazka, Michal. / Cloning, expression and genomic structure of human LMX1A, and variant screening in Pima Indians. In: Gene. 2002 ; Vol. 290, No. 1-2. pp. 217-225.
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