Cloning efficiency of cultured human tumor cell lines measured with the use of a coulter particle counter

Bonni J. Hazelton; Pamela M. Torrance; Stephen L. George; Peter J. Houghton

doi:10.1093/jnci/73.3.555

Cloning efficiency of cultured human tumor cell lines measured with the use of a coulter particle counter

Bonni J. Hazelton, Pamela M. Torrance, Stephen L. George, Peter J. Houghton

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A technique is described by which colony formation in agarose may be rapidly and reproducibly determined with the use of a modified Coulter particle counter (CPC). The cloning efficiency of RD human rhabdomyosarcoma cells after exposure to vincristine sulfate or cisplatin has been compared with the CPC method or by conventional visual counting. These techniques give very similar results. In combination with a Coulter Channelyzer, the CPC technique can be used to evaluate drug effects in colony sizes from a median of 20 to greater than 290 cells. The integrity of colonies formed by 3 colon carcinoma cell lines was examined to determine if this method could be applied to other systems.

Original language	English (US)
Pages (from-to)	555-563
Number of pages	9
Journal	Journal of the National Cancer Institute
Volume	73
Issue number	3
DOIs	https://doi.org/10.1093/jnci/73.3.555
State	Published - Jan 1 1984
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

Access to Document

10.1093/jnci/73.3.555

Cite this

@article{7e470f4e6ff24e618f5b892d8ed48c21,

title = "Cloning efficiency of cultured human tumor cell lines measured with the use of a coulter particle counter",

abstract = "A technique is described by which colony formation in agarose may be rapidly and reproducibly determined with the use of a modified Coulter particle counter (CPC). The cloning efficiency of RD human rhabdomyosarcoma cells after exposure to vincristine sulfate or cisplatin has been compared with the CPC method or by conventional visual counting. These techniques give very similar results. In combination with a Coulter Channelyzer, the CPC technique can be used to evaluate drug effects in colony sizes from a median of 20 to greater than 290 cells. The integrity of colonies formed by 3 colon carcinoma cell lines was examined to determine if this method could be applied to other systems.",

author = "Hazelton, {Bonni J.} and Torrance, {Pamela M.} and George, {Stephen L.} and Houghton, {Peter J.}",

note = "Funding Information: I Received June 16, 1983; revised March 28, 1984; accepted April 24, 1984. 2 Supported by grants CH·156 and IN·99I from the American Cancer Society and by the American, Lebanese, Syrian Associated Charities. 3 Division of Biochemical and Clinical Pharmacology, St. Jude Children's Research Hospital, 332 N. Lauderdale, P.O. Box 318, Memphis, Tenn. 38101. 4 Division of Biostatistics, St. Jude Children's Research Hospital. 5 Address reprint requests to Dr. Houghton.",

year = "1984",

month = jan,

day = "1",

doi = "10.1093/jnci/73.3.555",

language = "English (US)",

volume = "73",

pages = "555--563",

journal = "Journal of the National Cancer Institute",

issn = "0027-8874",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Cloning efficiency of cultured human tumor cell lines measured with the use of a coulter particle counter

AU - Hazelton, Bonni J.

AU - Torrance, Pamela M.

AU - George, Stephen L.

AU - Houghton, Peter J.

N1 - Funding Information: I Received June 16, 1983; revised March 28, 1984; accepted April 24, 1984. 2 Supported by grants CH·156 and IN·99I from the American Cancer Society and by the American, Lebanese, Syrian Associated Charities. 3 Division of Biochemical and Clinical Pharmacology, St. Jude Children's Research Hospital, 332 N. Lauderdale, P.O. Box 318, Memphis, Tenn. 38101. 4 Division of Biostatistics, St. Jude Children's Research Hospital. 5 Address reprint requests to Dr. Houghton.

PY - 1984/1/1

Y1 - 1984/1/1

N2 - A technique is described by which colony formation in agarose may be rapidly and reproducibly determined with the use of a modified Coulter particle counter (CPC). The cloning efficiency of RD human rhabdomyosarcoma cells after exposure to vincristine sulfate or cisplatin has been compared with the CPC method or by conventional visual counting. These techniques give very similar results. In combination with a Coulter Channelyzer, the CPC technique can be used to evaluate drug effects in colony sizes from a median of 20 to greater than 290 cells. The integrity of colonies formed by 3 colon carcinoma cell lines was examined to determine if this method could be applied to other systems.

AB - A technique is described by which colony formation in agarose may be rapidly and reproducibly determined with the use of a modified Coulter particle counter (CPC). The cloning efficiency of RD human rhabdomyosarcoma cells after exposure to vincristine sulfate or cisplatin has been compared with the CPC method or by conventional visual counting. These techniques give very similar results. In combination with a Coulter Channelyzer, the CPC technique can be used to evaluate drug effects in colony sizes from a median of 20 to greater than 290 cells. The integrity of colonies formed by 3 colon carcinoma cell lines was examined to determine if this method could be applied to other systems.

UR - http://www.scopus.com/inward/record.url?scp=0021193102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021193102&partnerID=8YFLogxK

U2 - 10.1093/jnci/73.3.555

DO - 10.1093/jnci/73.3.555

M3 - Article

C2 - 6590907

AN - SCOPUS:0021193102

SN - 0027-8874

VL - 73

SP - 555

EP - 563

JO - Journal of the National Cancer Institute

JF - Journal of the National Cancer Institute

IS - 3

ER -

Cloning efficiency of cultured human tumor cell lines measured with the use of a coulter particle counter

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this