Cloning, characterization, and tissue expression pattern of mouse Nma/BAMBI during odontogenesis

C. Knight, D. Simmons, T. T. Gu, J. Gluhak-Heinrich, D. Pavlin, M. Zeichner-David, M. MacDougall

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Degenerate oligonucleotides to consensus serine kinase functional domains previously identified a novel, partial rabbit tooth cDNA (Zeichner-David et al., 1992) that was used in this study to identify a full-length mouse clone. A 1390-base-pair cDNA clone was isolated encoding a putative 260-amino-acid open reading frame containing a hydrophobic 25-amino-acid potential transmembrane domain. This clone shares some homology with the TGF-β type I receptor family, but lacks the intracellular kinase domain. DNA database analysis revealed that this clone has 86% identity to a newly isolated human gene termed non-metastatic gene A and 80% identity to a Xenopus cDNA clone termed BMP and activin membrane bound inhibitor. Here we report the mouse Nma/BAMBI cDNA sequence, the tissue expression pattern, and confirmed expression in dental cell lines. This study demonstrates that Nma/BAMBI is a highly conserved protein across species and is expressed at high levels during odontogenesis.

Original languageEnglish (US)
Pages (from-to)1895-1902
Number of pages8
JournalJournal of Dental Research
Volume80
Issue number10
DOIs
StatePublished - Jan 1 2001

ASJC Scopus subject areas

  • Dentistry(all)

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