Cloning and molecular characterization of a human ortholog of Monodelphis TRAPD in ultraviolet B-induced melanoma

Zhiqiang Wang, John L. VandeBerg

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The human ortholog of Monodelphis translocon-associated protein δ subunit gene (TRAPD) was identified as a differentially expressed transcript using the differential display technique, and the two phenotypically distinct opossum melanoma cell lines TD6b and TD15L2 as the tissue resource. The full-length cDNA of TRAPD that encodes a 144 amino acid protein was characterized. In addition, a relative quantitative reverse transcription- polymerase chain reaction approach was used to analyse the expression patterns of TRAPD during different stages of tumour progression. TRAPD was upregulated in tumours of advanced stages. The results of this study implicate TRAPD as a candidate gene with potential functions that might be associated with ultraviolet-induced melanomagenesis and metastasis.

Original languageEnglish (US)
Pages (from-to)107-114
Number of pages8
JournalMelanoma Research
Volume14
Issue number2
DOIs
StatePublished - Apr 2004
Externally publishedYes

Fingerprint

Monodelphis
Molecular Cloning
Melanoma
Opossums
Protein Subunits
Genes
Reverse Transcription
Neoplasms
Complementary DNA
Neoplasm Metastasis
Amino Acids
Cell Line
Polymerase Chain Reaction
Proteins

Keywords

  • Gene expression
  • Metastasis
  • Monodelphis
  • TRAPD
  • Ultraviolet B-induced melanoma

ASJC Scopus subject areas

  • Cancer Research
  • Dermatology

Cite this

Cloning and molecular characterization of a human ortholog of Monodelphis TRAPD in ultraviolet B-induced melanoma. / Wang, Zhiqiang; VandeBerg, John L.

In: Melanoma Research, Vol. 14, No. 2, 04.2004, p. 107-114.

Research output: Contribution to journalArticle

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