Cloning and molecular characterization of a human ortholog of Monodelphis TRAPD in ultraviolet B-induced melanoma

Zhiqiang Wang, John L. VandeBerg

    Research output: Contribution to journalArticlepeer-review

    4 Scopus citations

    Abstract

    The human ortholog of Monodelphis translocon-associated protein δ subunit gene (TRAPD) was identified as a differentially expressed transcript using the differential display technique, and the two phenotypically distinct opossum melanoma cell lines TD6b and TD15L2 as the tissue resource. The full-length cDNA of TRAPD that encodes a 144 amino acid protein was characterized. In addition, a relative quantitative reverse transcription- polymerase chain reaction approach was used to analyse the expression patterns of TRAPD during different stages of tumour progression. TRAPD was upregulated in tumours of advanced stages. The results of this study implicate TRAPD as a candidate gene with potential functions that might be associated with ultraviolet-induced melanomagenesis and metastasis.

    Original languageEnglish (US)
    Pages (from-to)107-114
    Number of pages8
    JournalMelanoma Research
    Volume14
    Issue number2
    DOIs
    StatePublished - Apr 1 2004

    Keywords

    • Gene expression
    • Metastasis
    • Monodelphis
    • TRAPD
    • Ultraviolet B-induced melanoma

    ASJC Scopus subject areas

    • Oncology
    • Dermatology
    • Cancer Research

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