Cloning and functional expression of CC CKR5, a human monocytg CC chemokine receptor selective for MIP-1α, MIP-1β, and RANTES

Christophe Combadiere, Sunil K. Ahuja, H. Lee Tiffany, Philip M. Murphy

Research output: Contribution to journalArticle

251 Scopus citations

Abstract

We have cloned a human cDNA for a novel CC chemokine receptor (CC CKR) designated CC CKR5 that has 48-75% amino acid identity to other CC CKRs. CC = CKR5 mRNA was detected constitutively in primary adherent monocytes but not in primary neutrophils or eosinophils. Macrophage inflammatory protein-1α (MIP-1α), MIP-1β, and RANTES were all potent agonists for CC CKR5 (EC50 = 3-30 nM) when calcium flux was measured in transfected HEK 293 cells, yet the apparent binding affinities of the corresponding iodinated chemokines to intact cells expressing the receptor were low (IC50 ~100 nM). The calcium flux responses were completely blocked by treatment of transfected cells with pertussis toxin. These data suggest that CC CKR5 is a G(i)-coupled receptor that may mediate monocyte responses to MIP-1α, MIP-1β, and RANTES.

Original languageEnglish (US)
Pages (from-to)147-152
Number of pages6
JournalJournal of Leukocyte Biology
Volume60
Issue number1
DOIs
StatePublished - Jul 1996

Keywords

  • Chemotaxis
  • G protein
  • Inflammation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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