Cloning and functional expression of a human eosinophil CC chemokine receptor

C. Combadiere, S. K. Ahuja, P. M. Murphy

Research output: Contribution to journalArticle

211 Scopus citations

Abstract

Eosinophils undergo chemotaxis, degranulate, and exhibit [Ca2+](i) changes in response to the human CC chemokines macrophage inflammatory protein (MIP)-1α, regulated on activation, normal T expressed and secreted (RANTES), and monocyte chemoattractant protein-3 (MCP-3), but the receptors involved have not been defined. We have isolated a human cDNA encoding the first eosinophil-selective chemokine receptor, designated CC chemokine receptor 3 (CC CKR3). CC CKR3 is a seven-transmembrane domain G protein- coupled receptor most closely related to the previously reported monocyte- and neutrophil-selective receptor CC CKR1 (also known as the MIP-1α/RANTES receptor). When [Ca2+](i) changes were monitored in stably transfected human embryonic kidney 293 cells, MIP-1α and RANTES were both potent agonists for CC CKR3 and CC CKR1. However, MIP-1β was also an agonist for CC CKR3 but not CC CKR1; MCP-3 was an agonist for CC CKR1 but not CC CKR3. CC CKR3 may be one of the host factors responsible for selective recruitment of eosinophils to sites of inflammation.

Original languageEnglish (US)
Pages (from-to)16491-16494
Number of pages4
JournalJournal of Biological Chemistry
Volume270
Issue number28
DOIs
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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