Cloning and expression of three rabbit kidney cDNAs encoding lauric acid ω-hydroxylases

Eric F. Johnson, Diana L. Walker, Keith J. Griffin, Joan E. Clark, Richard T. Okita, A. Scott Muerhoff, Bettie Sue Masters

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

cDNAs encoding three cytochrome P-450 enzymes were cloned from a rabbit kidney cDNA library. These three cDNAs exhibit >90% nucleotide sequence identity across the coding region. This degree of sequence identity is also seen with P450IVA4, an enzyme that catalyzes the ω-hydroxylation of prostaglandins and that is elevated during pregnancy and induced by progesterone in rabbit lung. The 3′ untranslated regions of the three cDNAs display very little sequence identity, suggesting that they are the products of distinct genes. The predicted amino acid sequences derived from each cDNA and for P450IVA4 exhibit about 85% identity. Each cDNA was inserted into an expression vector for transient transfection of COS-1 cells. The transfected cells each expressed a protein recognized by antibodies to P450IVA4. Microsomes isolated from the cells transfected with each cDNA efficiently catalyzed the ω-hydroxylation of lauric acid with rates that greatly exceed that catalyzed by microsomes isolated from the host cell line. One of the cDNAs encodes an enzyme that ω-hydroxylates prostaglandin A1; however, the specific activity was 2 orders of magnitude lower than that for lauric acid. Our results indicate that the substrate selectivity of the kidney P-450s encoded by these cDNAs is distinct from that of the lung P450IVA4 and that multiple enzymes comprise P-450 class IVA in the rabbit.

Original languageEnglish (US)
Pages (from-to)873-879
Number of pages7
JournalBiochemistry
Volume29
Issue number4
StatePublished - 1990
Externally publishedYes

Fingerprint

Cytochrome P-450 CYP4A
Cloning
Organism Cloning
Complementary DNA
Rabbits
Kidney
lauric acid
Hydroxylation
Microsomes
Cytochrome P-450 Enzyme System
Lung
COS Cells
3' Untranslated Regions
Enzymes
Gene Library
Prostaglandins
Transfection
Progesterone
Amino Acid Sequence
Nucleotides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Johnson, E. F., Walker, D. L., Griffin, K. J., Clark, J. E., Okita, R. T., Muerhoff, A. S., & Masters, B. S. (1990). Cloning and expression of three rabbit kidney cDNAs encoding lauric acid ω-hydroxylases. Biochemistry, 29(4), 873-879.

Cloning and expression of three rabbit kidney cDNAs encoding lauric acid ω-hydroxylases. / Johnson, Eric F.; Walker, Diana L.; Griffin, Keith J.; Clark, Joan E.; Okita, Richard T.; Muerhoff, A. Scott; Masters, Bettie Sue.

In: Biochemistry, Vol. 29, No. 4, 1990, p. 873-879.

Research output: Contribution to journalArticle

Johnson, EF, Walker, DL, Griffin, KJ, Clark, JE, Okita, RT, Muerhoff, AS & Masters, BS 1990, 'Cloning and expression of three rabbit kidney cDNAs encoding lauric acid ω-hydroxylases', Biochemistry, vol. 29, no. 4, pp. 873-879.
Johnson EF, Walker DL, Griffin KJ, Clark JE, Okita RT, Muerhoff AS et al. Cloning and expression of three rabbit kidney cDNAs encoding lauric acid ω-hydroxylases. Biochemistry. 1990;29(4):873-879.
Johnson, Eric F. ; Walker, Diana L. ; Griffin, Keith J. ; Clark, Joan E. ; Okita, Richard T. ; Muerhoff, A. Scott ; Masters, Bettie Sue. / Cloning and expression of three rabbit kidney cDNAs encoding lauric acid ω-hydroxylases. In: Biochemistry. 1990 ; Vol. 29, No. 4. pp. 873-879.
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