@article{8388246814e844aaa89ed39dc1486b06,
title = "Cloning and characterization of the lanosterol 14α-demethylase (ERG11) gene in Cryptococcus neoformans",
abstract = "The ergosterol pathway in fungal pathogens is an attractive antimicrobial target because it is unique from the major sterol (cholesterol) producing pathway in humans. Lanosterol 14α-demethylase is the target for a major class of antifungals, the azoles. In this study we have isolated the gene for this enzyme from Cryptococcus neoformans. The gene, ERG11, was recovered using degenerate PCR with primers designed with a novel algorithm called CODEHOP. Sequence analysis of Erg11p identified a highly conserved region typical of the cytochrome P450 class of mono-oxygenases. The gene was present in single copy in the genome and mapped to one end of the largest chromosome. Comparison of the protein sequence to a number of major human fungal pathogen Erg11p homologs revealed that the C. neoformans protein was highly conserved, and most closely related to the Erg11p homologs from other basidiomycetes. Functional studies demonstrated that the gene could complement a Saccharomyces cerevisiae erg11 mutant, which confirmed the identity of the C. neoformans gene.",
keywords = "Candida, Coccidioides, Codehop, Histoplasma, Lanosterol demethylase",
author = "Revankar, {S. G.} and J. Fu and Rinaldi, {M. G.} and Kelly, {S. L.} and Kelly, {D. E.} and Lamb, {D. C.} and Keller, {S. M.} and Wickes, {B. L.}",
note = "Funding Information: Preliminary sequence data for Cryptococcus neoformans and Coccidioides immitis was obtained from The Institute for Genomic Research (TIGR) through the website at http://www.tigr.org . Sequencing of these fungi was accomplished with support from the National Institute of Allergy and Infectious Diseases under Grants U01AI48594-01 and U01AI50910, respectively. The Histoplasma capsulatum sequence was obtained from the Washington University Genome Sequencing Center ( http://genome.wustl.edu/projects/hcapsulatum/ ) and is funded by U01AI50934 from the National Institute of Allergy and Infectious Diseases. The Coprinus cinereus sequencing project is part of the fungal genome initiative and is being sequenced at the Broad Institute, Center for Genome Research, at the Massachusetts Institute of Technology ( http://www.broad.mit.edu ). The Phanerochaete chrysosporium genome sequence ( http://genome.jgi-psf.org/whiterot1/whiterot1.home.html ) was performed under the auspices of the US Department of Energy{\textquoteright}s Office of Science, Biological and Environmental Research Program and by the University of California, Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48, Lawrence Berkeley National Laboratory under Contract No. DE-AC03-76SF00098, and Los Alamos National Laboratory under Contract No. W-7405-ENG-36. We thank Nicola Rolley for technical assistance and the MRC, BBSRC, and the Wolfson Foundation for financial support. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",
year = "2004",
month = nov,
day = "12",
doi = "10.1016/j.bbrc.2004.09.112",
language = "English (US)",
volume = "324",
pages = "719--728",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",
}