Cloning and characterization of Exodus, a novel β-chemokine

Robert Hromas, Patrick W. Gray, David Chantry, Ronald Godiska, Mitchell Krathwohl, Kenneth Fife, Graeme I. Bell, Jun Takeda, Susan Aronica, Michael Gordon, Scott Cooper, Hal E. Broxmeyer, Michael J. Klemsz

Research output: Contribution to journalArticle

166 Scopus citations

Abstract

Chemokines are a family of related proteins that regulate leukocyte infiltration into inflamed tissue. Some chemokines such as MIP-1 α also inhibit hematopoietic progenitor cell proliferation. Recently, three chemokines, MIP-1 α, MIP-1 β, and RANTES, have been found to significantly decrease human immunodeficiency virus production from infected T cells. We report here the cloning and characterization of a novel human chemokine termed Exodus for its chemotactic properties. This novel chemokine is distantly related to other chemokines (28% homology with MIP-1 α) and shares several biological activities. Exodus is expressed preferentially in lymphocytes and monocytes, and its expression is markedly upregulated by mediators of inflammation such as tumor necrosis factor or lipopolysaccharide. Purified synthetic Exodus was found to inhibit proliferation of myeloid progenitors in colony formation assays. Exodus also stimulated chemotaxis of peripheral blood mononuclear cells. The sequence homology, expression, and biological activity indicate that Exodus represents a novel divergent β-chemokine.

Original languageEnglish (US)
Pages (from-to)3315-3322
Number of pages8
JournalBlood
Volume89
Issue number9
StatePublished - May 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Hromas, R., Gray, P. W., Chantry, D., Godiska, R., Krathwohl, M., Fife, K., Bell, G. I., Takeda, J., Aronica, S., Gordon, M., Cooper, S., Broxmeyer, H. E., & Klemsz, M. J. (1997). Cloning and characterization of Exodus, a novel β-chemokine. Blood, 89(9), 3315-3322.