Clone B7 cells have a single copy of SIVsmB7 integrated in chromosome 20

I. Martínez, L. Giavedoni, E. Kraiselburd

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Summary. B7 is the designation of a cell clone derived from the human cell line CEMx174, which was infected with SIVsmH3 clone. B7 cells chronically produce high quantities of non-infectious virus-like particles (VLP) denominated SIVsmB7. Here we report the molecular characterization of the B7 cell line. We found that B7 cells have a single copy of the SIVsmB7 provirus integrated in a noncoding region of chromosome 20 (nt 24,957 of clone RP5-963K23 on human chromosome 20q 13.11-13.2). Similarly to HIV and SIVmac, we show that integration of SIVsm results in a characteristic five base pair sequence repeat of host DNA that flanks the proviral DNA genome. Since the SIVsmB7 genome has a deletion in the IN coding sequence, the generation of this defective proviral genome most likely occurred during a faulty process of reverse transcription. Thus, these studies reveal the molecular clonality of the SIVsmB7 VLP produced by B7 cells. These genetically homogeneous VLP are useful reagents for vaccine development. In addition, these particles have been used by others (Montelaro et al.) to study the maturation of immune system responses to SIV infection.

Original languageEnglish (US)
Pages (from-to)217-223
Number of pages7
JournalArchives of Virology
Volume147
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 20
Virion
Clone Cells
Genome
Cell Line
Proviruses
DNA
Human Chromosomes
Base Pairing
Reverse Transcription
Immune System
Vaccines
HIV
Infection

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Clone B7 cells have a single copy of SIVsmB7 integrated in chromosome 20. / Martínez, I.; Giavedoni, L.; Kraiselburd, E.

In: Archives of Virology, Vol. 147, No. 1, 2002, p. 217-223.

Research output: Contribution to journalArticle

Martínez, I. ; Giavedoni, L. ; Kraiselburd, E. / Clone B7 cells have a single copy of SIVsmB7 integrated in chromosome 20. In: Archives of Virology. 2002 ; Vol. 147, No. 1. pp. 217-223.
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