CLK-1/Coq7p is a DMQ mono-oxygenase and a new member of the di-iron carboxylate protein family

Shane Rea

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Strains of Caenorhabditis elegans mutant for clk-1 exhibit a 20-40% increase in mean lifespan. clk-1 encodes a mitochondrial protein thought to be either an enzyme or regulatory molecule acting within the ubiquinone biosynthesis pathway. Here CLK-1 is shown to be related to the ubiquinol oxidase, alternative oxidase, and belong to the functionally diverse di-iron-carboxylate protein family which includes bacterioferritin and methane mono-oxygenase. Construction and analysis of a homology model indicates CLK-1 is a 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone mono-oxygenase as originally predicted. Analysis of known CLK-1/Coq7p mutations also supports this notion. These findings raise the possibility of developing CLK-1-specific inhibitors to test for lifespan extension in higher organisms.

Original languageEnglish (US)
Pages (from-to)389-394
Number of pages6
JournalFEBS Letters
Volume509
Issue number3
DOIs
StatePublished - Dec 14 2001

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Keywords

  • Aging
  • Alternative oxidase
  • Bacterioferritin
  • Clk-1
  • Gerontogene
  • Senescence

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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