Clinical performance of a point-of-care Coccidioides antibody test in dogs

Krystle L. Reagan, Ian McHardy, George R. Thompson, Jane E. Sykes

Research output: Contribution to journalArticlepeer-review


Background: Point-of-care (POC) Coccidioides antibody assays may provide veterinarians with rapid and accurate diagnostic information. Objectives: To determine the agreement of a POC lateral flow assay (LFA), sona Coccidioides (IMMY, Norman, Oklahoma) with the current diagnostic standard, the immunodiffusion assay (agar gel immunodiffusion [AGID]; Coccidioidomycosis Serology Laboratory, University of California, Davis, California). Animals: Forty-eight sera specimens from 48 dogs. Methods: Sera specimens were collected from client-owned dogs that had a clinical suspicion for coccidioidomycosis. Animals were classified as Coccidioides antibody-positive (n = 36) based on a positive AGID or Coccidioides antibody-negative (n = 12) based on a negative AGID. The performance of the LFA assay was determined by comparing results to AGID results. Results: The LFA assay demonstrated agreement in 32 of 36 Coccidioides antibody-positive specimens and 12 of 12 Coccidioides antibody-negative specimens, resulting in a positive percentage agreement of 88.9% (95% confidence interval [CI], 74.7-95.6%) and negative percentage agreement of 100% (95% CI, 75.8-100%) as compared to AGID. A receiver operator characteristic curve was constructed, and the area under the curve was 0.944 (CI, 0.880-1.000). Conclusion and Clinical importance: This LFA is a rapid alternative to the traditional AGID. The LFA provides excellent predictive value for positive results. Positive agreement was lower in dogs with low AGID titers; therefore, confirmatory testing is recommended if a high index of suspicion exists.

Original languageEnglish (US)
Pages (from-to)965-969
Number of pages5
JournalJournal of Veterinary Internal Medicine
Issue number2
StatePublished - Mar 1 2021
Externally publishedYes


  • diagnostic test
  • fungal
  • lateral flow
  • veterinary

ASJC Scopus subject areas

  • veterinary(all)


Dive into the research topics of 'Clinical performance of a point-of-care Coccidioides antibody test in dogs'. Together they form a unique fingerprint.

Cite this