Clinical evaluation of demineralized freeze-dried bone allograft with and without enamel matrix derivative for the treatment of periodontal osseous defects in humans

Matt J. Hoidal, Brently A. Grimard, Michael P. Mills, John D. Schoolfield, James T. Mellonig, Brian L. Mealey

Research output: Contribution to journalArticle

25 Scopus citations


Background: Beneficial clinical effects have been demonstrated with the addition of enamel matrix derivative (EMD) to demineralized freeze-dried bone allograft (DFDBA) compared to EMD alone. The purpose of this study was to evaluate the effectiveness of DFDBA combined with EMD compared to DFDBA alone in the treatment of intraosseous defects of chronic periodontitis. Methods: Thirty-two patients with 41 intrabony defects ‡3 mm were randomly assigned to one of two treatment groups. Intrabony defects were treated with DFDBA alone or in combination with EMD. Soft tissue measurements included probing depth (PD), gingival recession, and clinical attachment level (CAL). Hard tissue measurements included height of the alveolar crest, defect depth, and defect morphology. Following 6 months of healing, all measurements were repeated with the use of a surgical reentry procedure on 29 patients. Data were analyzed to determine PD reduction, CAL gain, change in recession, crestal resorption, defect fill, defect resolution, percentage of defect fill, and percentage of defect resolution. Results: Analysis of soft and hard tissue measurements demonstrated a statistically significant difference from baseline within each group (P <0.001); however, there was no statistically significant difference between the groups. Conclusion: Both treatments were shown to be safe and effective therapy for periodontal defects; however, the addition of EMD to DFDBA provided no statistically significant improvement to the soft and hard tissue parameters measured.

Original languageEnglish (US)
Pages (from-to)2273-2280
Number of pages8
JournalJournal of periodontology
Issue number12
StatePublished - Dec 1 2008



  • Bone regeneration
  • Demineralized freeze-dried bone allograft
  • Enamel matrix derivative
  • Periodontal regeneration
  • Periodontitis
  • Tissue engineering

ASJC Scopus subject areas

  • Periodontics

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