Cleavage site mapping and substrate-specificity of Leishmaniavirus 2-1 capsid endoribonuclease activity

Kyle J. MacBeth, Young Tae Ro, Lee Gehrke, Jean L. Patterson

    Research output: Contribution to journalArticlepeer-review

    6 Scopus citations

    Abstract

    The Leishmaniavirus capsid protein possesses an RNA endoribonuclease activity that cleaves viral positive-sense RNA at a specific, single site within the 5' untranslated region. The site of cleavage in LRV1-4 RNA was previously mapped to nucleotide 320 of the LRV1-4 genome. Here we show that an LRV2-1-derived substrate RNA transcript is also cleaved at a single site in an in vitro cleavage assay with LRV2-1 virions. Precise RNA cleavage site mapping in this divergent Old World virus, LRV2-1, confirms that cleavage is occurring within a region of homology to the LRV1 isolates. Substrate RNA transcripts possessing viral sequences from LRV1-4 or LRV2-1 genomes were assayed for susceptibility to cleavage by the cognate and noncognate capsid endoribonucleases to determine the level of substrate specificity.

    Original languageEnglish (US)
    Pages (from-to)193-200
    Number of pages8
    JournalJournal of Biochemistry
    Volume122
    Issue number1
    DOIs
    StatePublished - Jul 1997

    Keywords

    • Capsid protein
    • Cleavage site mapping
    • Leishmania RNA virus
    • Site-specific RNA endonuclease activity
    • Substrate specificity

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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