Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation

Rui J Sousa, Hsien Shun Liao, Jorge Cuéllar, Suping Jin, José M. Valpuesta, Albert J. Jin, Eileen M Lafer

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28 Scopus citations

Abstract

Hsp70s use ATP hydrolysis to disrupt protein-protein associations and to move macromolecules. One example is the Hsc70- mediated disassembly of the clathrin coats that form on vesicles during endocytosis. Here, we exploited the exceptional features of these coats to test three models - Brownian ratchet, power-stroke and entropic pulling - proposed to explain how Hsp70s transform their substrates. Our data rule out the ratchet and power-stroke models and instead support a collision-pressure mechanism whereby collisions between clathrin-coat walls and Hsc70s drive coats apart. Collision pressure is the complement to the pulling force described in the entropic pulling model. We also found that self-association augments collision pressure, thereby allowing disassembly of clathrin lattices that have been predicted to be resistant to disassembly. These results illuminate how Hsp70s generate the forces that transform their substrates.

Original languageEnglish (US)
Pages (from-to)821-829
Number of pages9
JournalNature Structural and Molecular Biology
Volume23
Issue number9
DOIs
StatePublished - Sep 1 2016

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ASJC Scopus subject areas

  • Structural Biology
  • Medicine(all)
  • Molecular Biology

Cite this

Sousa, R. J., Liao, H. S., Cuéllar, J., Jin, S., Valpuesta, J. M., Jin, A. J., & Lafer, E. M. (2016). Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation. Nature Structural and Molecular Biology, 23(9), 821-829. https://doi.org/10.1038/nsmb.3272