Abstract
The role of clathrin-mediated endocytosis in SV (synaptic vesicle) recycling has been studied by combining molecular biology, physiology and electron microscopy at the squid giant synapse. Procedures that prevent clathrin from assembling into membrane coats, such as impairment of binding of the AP180 and AP-2 adaptor proteins, completely prevent membrane budding during endocytosis. These procedures also reduce exocytosis, presumably an indirect effect of a reduction in the number of SVs following block of endocytosis. Disrupting the binding of auxilin to Hsc70 (heat-shock cognate 70) prevents clathrin-coated vesicles from uncoating and also disrupts SV recycling. Taken together, these results indicate that a clathrin-dependent pathway is the primary means of SV recycling at this synapse under physiological conditions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 68-72 |
| Number of pages | 5 |
| Journal | Biochemical Society Transactions |
| Volume | 34 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 2006 |
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Keywords
- Adaptor protein
- Clathrin
- Plasma membrane
- Squid giant synapse
- Synaptic vesicle
- Vesicle recycling
ASJC Scopus subject areas
- Biochemistry
Cite this
Clathrin and synaptic vesicle endocytosis : Studies at the squid giant synapse. / Augustine, G. J.; Morgan, J. R.; Villalba-Galea, C. A.; Jin, S.; Prasad, K.; Lafer, Eileen M.
In: Biochemical Society Transactions, Vol. 34, No. 1, 02.2006, p. 68-72.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clathrin and synaptic vesicle endocytosis
T2 - Studies at the squid giant synapse
AU - Augustine, G. J.
AU - Morgan, J. R.
AU - Villalba-Galea, C. A.
AU - Jin, S.
AU - Prasad, K.
AU - Lafer, Eileen M
PY - 2006/2
Y1 - 2006/2
N2 - The role of clathrin-mediated endocytosis in SV (synaptic vesicle) recycling has been studied by combining molecular biology, physiology and electron microscopy at the squid giant synapse. Procedures that prevent clathrin from assembling into membrane coats, such as impairment of binding of the AP180 and AP-2 adaptor proteins, completely prevent membrane budding during endocytosis. These procedures also reduce exocytosis, presumably an indirect effect of a reduction in the number of SVs following block of endocytosis. Disrupting the binding of auxilin to Hsc70 (heat-shock cognate 70) prevents clathrin-coated vesicles from uncoating and also disrupts SV recycling. Taken together, these results indicate that a clathrin-dependent pathway is the primary means of SV recycling at this synapse under physiological conditions.
AB - The role of clathrin-mediated endocytosis in SV (synaptic vesicle) recycling has been studied by combining molecular biology, physiology and electron microscopy at the squid giant synapse. Procedures that prevent clathrin from assembling into membrane coats, such as impairment of binding of the AP180 and AP-2 adaptor proteins, completely prevent membrane budding during endocytosis. These procedures also reduce exocytosis, presumably an indirect effect of a reduction in the number of SVs following block of endocytosis. Disrupting the binding of auxilin to Hsc70 (heat-shock cognate 70) prevents clathrin-coated vesicles from uncoating and also disrupts SV recycling. Taken together, these results indicate that a clathrin-dependent pathway is the primary means of SV recycling at this synapse under physiological conditions.
KW - Adaptor protein
KW - Clathrin
KW - Plasma membrane
KW - Squid giant synapse
KW - Synaptic vesicle
KW - Vesicle recycling
UR - http://www.scopus.com/inward/record.url?scp=32544434839&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=32544434839&partnerID=8YFLogxK
U2 - 10.1042/BST0340068
DO - 10.1042/BST0340068
M3 - Article
C2 - 16417485
AN - SCOPUS:32544434839
VL - 34
SP - 68
EP - 72
JO - Biochemical Society Transactions
JF - Biochemical Society Transactions
SN - 0300-5127
IS - 1
ER -