TY - JOUR
T1 - Citrobacter rodentium-induced colitis
T2 - A robust model to study mucosal immune responses in the gut
AU - Koroleva, Ekaterina P.
AU - Halperin, Sydney
AU - Gubernatorova, Ekaterina O.
AU - Macho-Fernandez, Elise
AU - Spencer, Cody M.
AU - Tumanov, Alexei V.
N1 - Funding Information:
This work was supported by the Crohn and Colitis Foundation of America SRA#294083 to A.V.T. A.V.T. was supported by Russian Scientific Fund # 14-50-00060 . We thank E. Leadbetter for the help with ELISA.
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Citrobacter rodentium is a natural mouse pathogen which reproducibly infects mice and causes intestinal disease. The C. rodentium model of infection is very useful for investigating host-pathogen immune interactions in the gut, and can also be used to understand the pathogenesis of several important human intestinal disorders, including Crohn's disease, ulcerative colitis, dysbiosis and colon tumorigenesis. Both innate and adaptive immune responses play a critical role in protection against C. rodentium. Here, we summarize the role of immune components in protection against C. rodentium and describe techniques for the analysis of innate and adaptive mucosal immune responses, including setting up the infection, analysis of colonic hyperplasia and bacterial dissemination, evaluation of antibody responses, and purification and analysis of intestinal epithelial and lymphoid cells.
AB - Citrobacter rodentium is a natural mouse pathogen which reproducibly infects mice and causes intestinal disease. The C. rodentium model of infection is very useful for investigating host-pathogen immune interactions in the gut, and can also be used to understand the pathogenesis of several important human intestinal disorders, including Crohn's disease, ulcerative colitis, dysbiosis and colon tumorigenesis. Both innate and adaptive immune responses play a critical role in protection against C. rodentium. Here, we summarize the role of immune components in protection against C. rodentium and describe techniques for the analysis of innate and adaptive mucosal immune responses, including setting up the infection, analysis of colonic hyperplasia and bacterial dissemination, evaluation of antibody responses, and purification and analysis of intestinal epithelial and lymphoid cells.
KW - Citrobacter rodentium
KW - Infection-induced colitis
KW - Mucosal model of inflammation
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U2 - 10.1016/j.jim.2015.02.003
DO - 10.1016/j.jim.2015.02.003
M3 - Article
C2 - 25702536
AN - SCOPUS:84929953466
SN - 0022-1759
VL - 421
SP - 61
EP - 72
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
ER -