Citrobacter rodentium-induced colitis: A robust model to study mucosal immune responses in the gut

Ekaterina P. Koroleva, Sydney Halperin, Ekaterina O. Gubernatorova, Elise Macho-Fernandez, Cody M. Spencer, Alexei V. Tumanov

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Citrobacter rodentium is a natural mouse pathogen which reproducibly infects mice and causes intestinal disease. The C. rodentium model of infection is very useful for investigating host-pathogen immune interactions in the gut, and can also be used to understand the pathogenesis of several important human intestinal disorders, including Crohn's disease, ulcerative colitis, dysbiosis and colon tumorigenesis. Both innate and adaptive immune responses play a critical role in protection against C. rodentium. Here, we summarize the role of immune components in protection against C. rodentium and describe techniques for the analysis of innate and adaptive mucosal immune responses, including setting up the infection, analysis of colonic hyperplasia and bacterial dissemination, evaluation of antibody responses, and purification and analysis of intestinal epithelial and lymphoid cells.

Original languageEnglish (US)
Pages (from-to)61-72
Number of pages12
JournalJournal of Immunological Methods
Volume421
DOIs
StatePublished - Jun 1 2015
Externally publishedYes

Keywords

  • Citrobacter rodentium
  • Infection-induced colitis
  • Mucosal model of inflammation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Medicine(all)

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