Circadian variation of β-adrenoceptor binding sites in the pineal gland of the Syrian hamster and prevention of the nocturnal reduction by light exposure or propranolol treatment

B. Pangerl, A. Pangerl, Russel J Reiter, D. J. Jones

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Abstract

Radioreceptor assays with (-)[125I]-iodopindolol (IPIN) were used to describe circadian variations of β-adrenoceptors in the pineal gland of male Syrian hamsters (Mesocricetus auratus). Single-point experiments (200 pM IPIN, 8-9 pineals pooled per time point) showed a significant circadian variation (p < 0.01) with maximal values (28.9 ± 1.8 fmol/mg protein, x̄ ± SEM, n = 7) between 07:00 and 02:30 h, and minimal values at 04:00 (7.2 ± 2.9 fmol/mg protein, n = 3), 8 h after darkness onset. Either exposure of animals to light at night or treating dark exposed hamsters with a β-adrenergic receptor blocker, propranolol, prevented the nocturnal drop in the number of β-adrenoceptors. Scatchard analysis of saturation isotherms at 02:30, 04:00 and 08:00 h (30-600 pM IPIN, one saturation experiment with 25 pineals pooled per time point) confirmed the circadian variation.

Original languageEnglish (US)
Pages (from-to)570-573
Number of pages4
JournalNeuroendocrinology
Volume49
Issue number6
StatePublished - 1989

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Pineal Gland
Mesocricetus
Propranolol
Adrenergic Receptors
Binding Sites
Light
Radioligand Assay
Adrenergic Antagonists
Darkness
Cricetinae
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

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title = "Circadian variation of β-adrenoceptor binding sites in the pineal gland of the Syrian hamster and prevention of the nocturnal reduction by light exposure or propranolol treatment",
abstract = "Radioreceptor assays with (-)[125I]-iodopindolol (IPIN) were used to describe circadian variations of β-adrenoceptors in the pineal gland of male Syrian hamsters (Mesocricetus auratus). Single-point experiments (200 pM IPIN, 8-9 pineals pooled per time point) showed a significant circadian variation (p < 0.01) with maximal values (28.9 ± 1.8 fmol/mg protein, x̄ ± SEM, n = 7) between 07:00 and 02:30 h, and minimal values at 04:00 (7.2 ± 2.9 fmol/mg protein, n = 3), 8 h after darkness onset. Either exposure of animals to light at night or treating dark exposed hamsters with a β-adrenergic receptor blocker, propranolol, prevented the nocturnal drop in the number of β-adrenoceptors. Scatchard analysis of saturation isotherms at 02:30, 04:00 and 08:00 h (30-600 pM IPIN, one saturation experiment with 25 pineals pooled per time point) confirmed the circadian variation.",
author = "B. Pangerl and A. Pangerl and Reiter, {Russel J} and Jones, {D. J.}",
year = "1989",
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volume = "49",
pages = "570--573",
journal = "Neuroendocrinology",
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T1 - Circadian variation of β-adrenoceptor binding sites in the pineal gland of the Syrian hamster and prevention of the nocturnal reduction by light exposure or propranolol treatment

AU - Pangerl, B.

AU - Pangerl, A.

AU - Reiter, Russel J

AU - Jones, D. J.

PY - 1989

Y1 - 1989

N2 - Radioreceptor assays with (-)[125I]-iodopindolol (IPIN) were used to describe circadian variations of β-adrenoceptors in the pineal gland of male Syrian hamsters (Mesocricetus auratus). Single-point experiments (200 pM IPIN, 8-9 pineals pooled per time point) showed a significant circadian variation (p < 0.01) with maximal values (28.9 ± 1.8 fmol/mg protein, x̄ ± SEM, n = 7) between 07:00 and 02:30 h, and minimal values at 04:00 (7.2 ± 2.9 fmol/mg protein, n = 3), 8 h after darkness onset. Either exposure of animals to light at night or treating dark exposed hamsters with a β-adrenergic receptor blocker, propranolol, prevented the nocturnal drop in the number of β-adrenoceptors. Scatchard analysis of saturation isotherms at 02:30, 04:00 and 08:00 h (30-600 pM IPIN, one saturation experiment with 25 pineals pooled per time point) confirmed the circadian variation.

AB - Radioreceptor assays with (-)[125I]-iodopindolol (IPIN) were used to describe circadian variations of β-adrenoceptors in the pineal gland of male Syrian hamsters (Mesocricetus auratus). Single-point experiments (200 pM IPIN, 8-9 pineals pooled per time point) showed a significant circadian variation (p < 0.01) with maximal values (28.9 ± 1.8 fmol/mg protein, x̄ ± SEM, n = 7) between 07:00 and 02:30 h, and minimal values at 04:00 (7.2 ± 2.9 fmol/mg protein, n = 3), 8 h after darkness onset. Either exposure of animals to light at night or treating dark exposed hamsters with a β-adrenergic receptor blocker, propranolol, prevented the nocturnal drop in the number of β-adrenoceptors. Scatchard analysis of saturation isotherms at 02:30, 04:00 and 08:00 h (30-600 pM IPIN, one saturation experiment with 25 pineals pooled per time point) confirmed the circadian variation.

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VL - 49

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EP - 573

JO - Neuroendocrinology

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