Chronic lymphocytic leukemia B cells can undergo somatic hypermutation and intraclonal immunoglobulin VHDJH gene diversification

Carmela Gurrieri, Peter McGuire, Hong Zan, Xiao Jie Yan, Andrea Cerutti, Emilia Albesiano, Steven L. Allen, Vincent Vinciguerra, Kanti R. Rai, Manlio Ferrarini, Paolo Casali, Nicholas Chiorazzi

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

Chronic lymphocytic leukemia (CLL) arises from the clonal expansion of a CD5+ B lymphocyte that is thought not to undergo intraclonal diversification. Using VHDJH cDNA single strand conformation polymorphism analyses, we detected intraclonal mobility variants in 11 of 18 CLL cases. cDNA sequence analyses indicated that these variants represented unique point-mutations (1-35/patient). In nine cases, these mutations were unique to individual submembers of the CLL clone, although in two cases they occurred in a large percentage of the clonal submembers and genealogical trees could be identified. The diversification process responsible for these changes led to single nucleotide changes that favored transitions over transversions, but did not target A nucleotides and did not have the replacement/silent nucleotide change characteristics of antigen-selected B cells. Intraclonal diversification did not correlate with the original mutational load of an individual CLL case in that diversification was as frequent in CLL cells with little or no somatic mutations as in those with considerable mutations. Finally, CLL B cells that did not exhibit intraclonal diversification in vivo could be induced to mutate their VHDJH genes in vitro after stimulation. These data indicate that a somatic mutation mechanism remains functional in CLL cells and could play a role in the evolution of the clone.

Original languageEnglish (US)
Pages (from-to)629-639
Number of pages11
JournalJournal of Experimental Medicine
Volume196
Issue number5
DOIs
StatePublished - Sep 2002

Keywords

  • B lymphocyte
  • Chronic lymphocytic leukemia
  • Ig gene
  • Somatic hypermutation
  • V gene diversification

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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