Chronic & infrequent opioid exposure suppresses IL-2r expression on rhesus monkey peripheral blood mononuclear cells following stimulation with pokeweed mttogen

Daniel J.J. Carr, Greg W. Carpenter, Hildegardo H. Garza, Charles P. France, Om Prakash

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

In the present investigation, infrequent and chronic (daily) exposure of rhesus monkeys to morphine was investigated for their effect on cytokine receptor expression, interleukin (IL)-2 production, and nuclear factor κPB (NFκPB) levels following stimulation with PWM. In a time-dependent manner, peripheral blood mononuclearcells (PBMCs) from both infrequent- and daily-morphine treated monkeys displayed significantly less (40 ± 7% IL-2 receptor compared to PBMCs from saline-treated controls. However, PBMCs from chronic opioid- and infrequent opioidtreated monkeys displayed similar levels of IL-4 and IL-7 receptors compared to saline-treated animals. Following stimulation with PWM, PBMCs from chronic opioid-treated monkeys produced elevated levels of IL-2 (870 ± 94 pg/ml) compared to IL-2 levels (463 ± 88 pg/ml) of PBMCs from saline-treated monkey. Likewise, PBMCs from chronic-morphine exposed monkeys had elevated levels (43% of NFκPB compared to PBMCs from saline-treated (control) monkeys following 72 hours in culture with PWM. Collectively, these observations suggest morphine regulates selective molecular events that manifest in biologically altered immune function including T cell activation and IL-2 production.

Original languageEnglish (US)
Pages (from-to)137-148
Number of pages12
JournalInternational Journal of Neuroscience
Volume81
Issue number3-4
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Keywords

  • Interleukin-2
  • Morphine
  • NFκPB
  • Nuclear factor κPB
  • Rhesus monkey
  • Tumor necrosis factor- α

ASJC Scopus subject areas

  • Neuroscience(all)

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