Chromosome assignment of genes encoding the α and Β subunits of glycoprotein hormones in man and mouse

Susan L. Naylor, William W. Chin, Howard M. Goodman, Peter A. Lalley, Karl Heinz Grzeschik, Alan Y. Sakaguchi

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

The chromosomal locations of the genes for the common α subunit of the glycoprotein hormones and the Β subunit of chorionic gonadotropin in humans and mice have been determined by restriction enzyme analysis of DNA isolated from somatic cell hybrids. The CGα gene (CGA), detected as a 15-kb BamHI fragment in human DNA by hybridization to CGα cDNA, segregated with the chromosome 6 enzyme markers ME1 (malic enzyme, soluble) and SOD2 (superoxide dismutase, mitchondrial) and an intact chromosome 6 in human-rodent hybrids. Cell hybrids containing portions of chromosome 6 allowed the localization of CGA to the q12 → q21 region. The >30- and 6.5-kb BamHI CGB fragments hybridizing to human CGΒ cDNA segregated concordantly with the human chromosome 19 marker enzymes PEPD (peptidase D) and GPI (glucose phosphate isomerase) and a normal chromosome 19 in karyotyped hybrids. A KpnI-HindIII digest of cell hybrid DNAs indicated that the multiple copies of the CGΒ gene are all located on human chromosome 19. In the mouse, the α subunit gene, detected by a mouse thyrotropin (TSH) α subunit probe, and the CGΒ-like sequences (CGΒ-LHΒ), detected by the human CGΒ cDNA probe, are on chromosomes 4 and 7, respectively.

Original languageEnglish (US)
Pages (from-to)757-770
Number of pages14
JournalSomatic Cell Genetics
Volume9
Issue number6
DOIs
StatePublished - Nov 1983
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Chromosome assignment of genes encoding the α and Β subunits of glycoprotein hormones in man and mouse'. Together they form a unique fingerprint.

  • Cite this