Chromosomal rearrangement in Candida stellatoidea results in a positive effect on phenotype

Brian L Wickes, J. E. Golin, K. J. Kwon-Chung

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

When type I Candida stellatoidea is plated onto sucrose agar at levels in excess of 108 cells, some isolates spontaneously form sucrose-positive colonies. These isolates do not display typical type I phenotypes but instead exhibit phenotypes intermediate between type I C. stellatoidea and C. albicans. Also, this phenotypic change only occurs in conjunction with a chromosomal rearrangement. These rearrangements have been studied in a strain naturally marked for methionine auxotrophy. Chromosome-size DNA bands separated by pulsed-field gel electrophoresis were probed with genes cloned from C. albicans. The hybridization pattern indicated that the genes on several chromosomes underwent extensive rearrangement.

Original languageEnglish (US)
Pages (from-to)1762-1771
Number of pages10
JournalInfection and Immunity
Volume59
Issue number5
StatePublished - 1991
Externally publishedYes

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Candida
Sucrose
Chromosomes
Phenotype
Pulsed Field Gel Electrophoresis
Methionine
Genes
Agar
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Chromosomal rearrangement in Candida stellatoidea results in a positive effect on phenotype. / Wickes, Brian L; Golin, J. E.; Kwon-Chung, K. J.

In: Infection and Immunity, Vol. 59, No. 5, 1991, p. 1762-1771.

Research output: Contribution to journalArticle

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