Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase

N. K. Honey; A. Y. Sakaguchi; C. Quinto; R. J. MacDonald; G. I. Bell; C. Craik; W. J. Rutter; S. L. Naylor

doi:10.1007/BF01535632

Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase

N. K. Honey, A. Y. Sakaguchi, C. Quinto, R. J. MacDonald, G. I. Bell, C. Craik, W. J. Rutter, S. L. Naylor

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The genes for the serine proteases trypsin, chymotrypsin B, and elastase were chromosomally assigned in man using cDNA probes that have been isolated from a rat pancreatic cDNA library. DNA from human × rodent somatic cell hybrids was cleaved with BamHI or EcoRI and analyzed by Southern filter hybridization methods for the segregation of the genes for trypsin-1 (TRY1), chymotrypsin B (CTRB), and elastase-1 (ELA1). TRY1 was assigned to human chromosome 7q22→qter, CTRB to chromosome 16, and ELA1 to chromosome 12. Although the three genes are members of the same gene family, they are dispersed over different chromosomes.

Original language	English (US)
Pages (from-to)	369-376
Number of pages	8
Journal	Somatic Cell and Molecular Genetics
Volume	10
Issue number	4
DOIs	https://doi.org/10.1007/BF01535632
State	Published - Jul 1984
Externally published	Yes

ASJC Scopus subject areas

Genetics
Cell Biology

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title = "Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase",

abstract = "The genes for the serine proteases trypsin, chymotrypsin B, and elastase were chromosomally assigned in man using cDNA probes that have been isolated from a rat pancreatic cDNA library. DNA from human × rodent somatic cell hybrids was cleaved with BamHI or EcoRI and analyzed by Southern filter hybridization methods for the segregation of the genes for trypsin-1 (TRY1), chymotrypsin B (CTRB), and elastase-1 (ELA1). TRY1 was assigned to human chromosome 7q22→qter, CTRB to chromosome 16, and ELA1 to chromosome 12. Although the three genes are members of the same gene family, they are dispersed over different chromosomes.",

author = "Honey, \{N. K.\} and Sakaguchi, \{A. Y.\} and C. Quinto and MacDonald, \{R. J.\} and Bell, \{G. I.\} and C. Craik and Rutter, \{W. J.\} and Naylor, \{S. L.\}",

year = "1984",

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doi = "10.1007/BF01535632",

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volume = "10",

pages = "369--376",

journal = "Somatic Cell and Molecular Genetics",

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T1 - Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase

AU - Honey, N. K.

AU - Sakaguchi, A. Y.

AU - Quinto, C.

AU - MacDonald, R. J.

AU - Bell, G. I.

AU - Craik, C.

AU - Rutter, W. J.

AU - Naylor, S. L.

PY - 1984/7

Y1 - 1984/7

N2 - The genes for the serine proteases trypsin, chymotrypsin B, and elastase were chromosomally assigned in man using cDNA probes that have been isolated from a rat pancreatic cDNA library. DNA from human × rodent somatic cell hybrids was cleaved with BamHI or EcoRI and analyzed by Southern filter hybridization methods for the segregation of the genes for trypsin-1 (TRY1), chymotrypsin B (CTRB), and elastase-1 (ELA1). TRY1 was assigned to human chromosome 7q22→qter, CTRB to chromosome 16, and ELA1 to chromosome 12. Although the three genes are members of the same gene family, they are dispersed over different chromosomes.

AB - The genes for the serine proteases trypsin, chymotrypsin B, and elastase were chromosomally assigned in man using cDNA probes that have been isolated from a rat pancreatic cDNA library. DNA from human × rodent somatic cell hybrids was cleaved with BamHI or EcoRI and analyzed by Southern filter hybridization methods for the segregation of the genes for trypsin-1 (TRY1), chymotrypsin B (CTRB), and elastase-1 (ELA1). TRY1 was assigned to human chromosome 7q22→qter, CTRB to chromosome 16, and ELA1 to chromosome 12. Although the three genes are members of the same gene family, they are dispersed over different chromosomes.

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Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase

Abstract

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