Chromatographic analysis of Vinca alkaloids in human neoplastic tissues and host (mouse) tissues after injection in vivo or after incubation in vitro

Janet A. Houghton; Pamela M. Torrance; Peter J. Houghton

doi:10.1016/0003-2697(83)90321-4

Chromatographic analysis of Vinca alkaloids in human neoplastic tissues and host (mouse) tissues after injection in vivo or after incubation in vitro

Janet A. Houghton
, Pamela M. Torrance
, Peter J. Houghton

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

A method for extracting from biological tissues vincristine, vinblastine, and their metabolites and analysis by high-performance liquid chromatography has been developed. After excision tissues are rapidly frozen in liquid nitrogen (<10 s) and powders are made under liquid N₂. Extraction of blood, plasma, or tissue powders was achieved using ethanol (95%) acidified to pH 4.9 with acetic acid. Extracts were analyzed using reverse-phase chromatography capable of separating Vinca alkaloids with substitutions on the vindoline or catharanthine moiety. This technique has been used to elucidate the metabolism of [³H]vincristine and [³H]vinblastine in vivo and in vitro.

Original language	English (US)
Pages (from-to)	450-454
Number of pages	5
Journal	Analytical Biochemistry
Volume	134
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(83)90321-4
State	Published - Oct 15 1983
Externally published	Yes

Keywords

HPLC analysis
Vinca alkaloids
extraction
metabolites
microsomal

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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10.1016/0003-2697(83)90321-4

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@article{ae2582b6c73e4834bd94476cb71a4df4,

title = "Chromatographic analysis of Vinca alkaloids in human neoplastic tissues and host (mouse) tissues after injection in vivo or after incubation in vitro",

abstract = "A method for extracting from biological tissues vincristine, vinblastine, and their metabolites and analysis by high-performance liquid chromatography has been developed. After excision tissues are rapidly frozen in liquid nitrogen (<10 s) and powders are made under liquid N2. Extraction of blood, plasma, or tissue powders was achieved using ethanol (95\%) acidified to pH 4.9 with acetic acid. Extracts were analyzed using reverse-phase chromatography capable of separating Vinca alkaloids with substitutions on the vindoline or catharanthine moiety. This technique has been used to elucidate the metabolism of [3H]vincristine and [3H]vinblastine in vivo and in vitro.",

keywords = "HPLC analysis, Vinca alkaloids, extraction, metabolites, microsomal",

author = "Houghton, \{Janet A.\} and Torrance, \{Pamela M.\} and Houghton, \{Peter J.\}",

note = "Funding Information: {\textquoteright} This work was supported by Grant CH-156 from the American Cancer Society and by ALSAC. 2 To whom reprint requests should be addressed. 3 Abbreviations used: VCR, vim\&tine; VLB, vinblastine; HPLC, high-performance liquid chromatography; EtOH, ethanol; MeOH, methanol.",

year = "1983",

month = oct,

day = "15",

doi = "10.1016/0003-2697(83)90321-4",

language = "English (US)",

volume = "134",

pages = "450--454",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

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TY - JOUR

T1 - Chromatographic analysis of Vinca alkaloids in human neoplastic tissues and host (mouse) tissues after injection in vivo or after incubation in vitro

AU - Houghton, Janet A.

AU - Torrance, Pamela M.

AU - Houghton, Peter J.

N1 - Funding Information: ’ This work was supported by Grant CH-156 from the American Cancer Society and by ALSAC. 2 To whom reprint requests should be addressed. 3 Abbreviations used: VCR, vim&tine; VLB, vinblastine; HPLC, high-performance liquid chromatography; EtOH, ethanol; MeOH, methanol.

PY - 1983/10/15

Y1 - 1983/10/15

N2 - A method for extracting from biological tissues vincristine, vinblastine, and their metabolites and analysis by high-performance liquid chromatography has been developed. After excision tissues are rapidly frozen in liquid nitrogen (<10 s) and powders are made under liquid N2. Extraction of blood, plasma, or tissue powders was achieved using ethanol (95%) acidified to pH 4.9 with acetic acid. Extracts were analyzed using reverse-phase chromatography capable of separating Vinca alkaloids with substitutions on the vindoline or catharanthine moiety. This technique has been used to elucidate the metabolism of [3H]vincristine and [3H]vinblastine in vivo and in vitro.

AB - A method for extracting from biological tissues vincristine, vinblastine, and their metabolites and analysis by high-performance liquid chromatography has been developed. After excision tissues are rapidly frozen in liquid nitrogen (<10 s) and powders are made under liquid N2. Extraction of blood, plasma, or tissue powders was achieved using ethanol (95%) acidified to pH 4.9 with acetic acid. Extracts were analyzed using reverse-phase chromatography capable of separating Vinca alkaloids with substitutions on the vindoline or catharanthine moiety. This technique has been used to elucidate the metabolism of [3H]vincristine and [3H]vinblastine in vivo and in vitro.

KW - HPLC analysis

KW - Vinca alkaloids

KW - extraction

KW - metabolites

KW - microsomal

UR - https://www.scopus.com/pages/publications/0021070391

UR - https://www.scopus.com/pages/publications/0021070391#tab=citedBy

U2 - 10.1016/0003-2697(83)90321-4

DO - 10.1016/0003-2697(83)90321-4

M3 - Article

C2 - 6650830

AN - SCOPUS:0021070391

SN - 0003-2697

VL - 134

SP - 450

EP - 454

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Chromatographic analysis of Vinca alkaloids in human neoplastic tissues and host (mouse) tissues after injection in vivo or after incubation in vitro

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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