Abstract
A method for extracting from biological tissues vincristine, vinblastine, and their metabolites and analysis by high-performance liquid chromatography has been developed. After excision tissues are rapidly frozen in liquid nitrogen (<10 s) and powders are made under liquid N2. Extraction of blood, plasma, or tissue powders was achieved using ethanol (95%) acidified to pH 4.9 with acetic acid. Extracts were analyzed using reverse-phase chromatography capable of separating Vinca alkaloids with substitutions on the vindoline or catharanthine moiety. This technique has been used to elucidate the metabolism of [3H]vincristine and [3H]vinblastine in vivo and in vitro.
Original language | English (US) |
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Pages (from-to) | 450-454 |
Number of pages | 5 |
Journal | Analytical Biochemistry |
Volume | 134 |
Issue number | 2 |
DOIs | |
State | Published - Oct 15 1983 |
Externally published | Yes |
Keywords
- HPLC analysis
- Vinca alkaloids
- extraction
- metabolites
- microsomal
ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
- Cell Biology