Chromatographic analysis of Vinca alkaloids in human neoplastic tissues and host (mouse) tissues after injection in vivo or after incubation in vitro

Janet A. Houghton, Pamela M. Torrance, Peter J Houghton

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A method for extracting from biological tissues vincristine, vinblastine, and their metabolites and analysis by high-performance liquid chromatography has been developed. After excision tissues are rapidly frozen in liquid nitrogen (<10 s) and powders are made under liquid N2. Extraction of blood, plasma, or tissue powders was achieved using ethanol (95%) acidified to pH 4.9 with acetic acid. Extracts were analyzed using reverse-phase chromatography capable of separating Vinca alkaloids with substitutions on the vindoline or catharanthine moiety. This technique has been used to elucidate the metabolism of [3H]vincristine and [3H]vinblastine in vivo and in vitro.

Original languageEnglish (US)
Pages (from-to)450-454
Number of pages5
JournalAnalytical Biochemistry
Volume134
Issue number2
DOIs
StatePublished - Oct 15 1983
Externally publishedYes

Fingerprint

Chromatographic analysis
Vinca Alkaloids
Chromatography
Vinblastine
Vincristine
Tissue
Powders
Injections
High performance liquid chromatography
Liquid nitrogen
Reverse-Phase Chromatography
Metabolites
Metabolism
Acetic Acid
Blood
Substitution reactions
Ethanol
Nitrogen
High Pressure Liquid Chromatography
Plasmas

Keywords

  • extraction
  • HPLC analysis
  • metabolites
  • microsomal
  • Vinca alkaloids

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Chromatographic analysis of Vinca alkaloids in human neoplastic tissues and host (mouse) tissues after injection in vivo or after incubation in vitro. / Houghton, Janet A.; Torrance, Pamela M.; Houghton, Peter J.

In: Analytical Biochemistry, Vol. 134, No. 2, 15.10.1983, p. 450-454.

Research output: Contribution to journalArticle

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