Chromatographic analysis of Vinca alkaloids in human neoplastic tissues and host (mouse) tissues after injection in vivo or after incubation in vitro

Janet A. Houghton, Pamela M. Torrance, Peter J. Houghton

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

A method for extracting from biological tissues vincristine, vinblastine, and their metabolites and analysis by high-performance liquid chromatography has been developed. After excision tissues are rapidly frozen in liquid nitrogen (<10 s) and powders are made under liquid N2. Extraction of blood, plasma, or tissue powders was achieved using ethanol (95%) acidified to pH 4.9 with acetic acid. Extracts were analyzed using reverse-phase chromatography capable of separating Vinca alkaloids with substitutions on the vindoline or catharanthine moiety. This technique has been used to elucidate the metabolism of [3H]vincristine and [3H]vinblastine in vivo and in vitro.

Original languageEnglish (US)
Pages (from-to)450-454
Number of pages5
JournalAnalytical Biochemistry
Volume134
Issue number2
DOIs
StatePublished - Oct 15 1983
Externally publishedYes

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Keywords

  • HPLC analysis
  • Vinca alkaloids
  • extraction
  • metabolites
  • microsomal

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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