Cholinergic mechanisms of cutaneous active vasodilation during heat stress in cystic fibrosis

Dean L Kellogg Jr, G. J. Hodges, C. R. Orozco, T. M. Phillips, J. L. Zhao, J. M. Johnson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To test the hypothesis that cutaneous active vasodilation in heat stress is mediated by a redundant cholinergic cotransmitter system, we examined the effects of atropine on skin blood flow (SkBF) increases during heat stress in persons with (CF) and without cystic fibrosis (non-CF). Vasoactive intestinal peptide (VIP) has been implicated as a mediator of cutaneous vasodilation in heat stress. VIP-containing cutaneous neurons are sparse in CF, yet SkBF increases during heat stress are normal. In CF, augmented ACh release or muscarinic receptor sensitivity could compensate for decreased VIP; if so, active vasodilation would be attenuated by atropine in CF relative to non-CF. Atropine was administered into skin by iontophoresis in seven CF and seven matched non-CF subjects. SkBF was monitored by laser-Doppler flowmetry (LDF) at atropine treated and untreated sites. Blood pressure [mean arterial pressure (MAP)] was monitored (Finapres), and cutaneous vascular conductance was calculated (CVC = LDF/MAP). The protocol began with a normothermic period followed by a 3-min cold stress and 30-45 min of heat stress. Finally, LDF sites were warmed to 42°C to effect maximal vasodilation. CVC was normalized to its site-specific maximum. During heat stress, CVC increased in both CF and non-CF (P < 0.01). CVC increases were attenuated by atropine in both groups (P < 0.01); however, the responses did not differ between groups (P = 0.99). We conclude that in CF there is not greater dependence on redundant cholinergic mechanisms for cutaneous active vasodilation than in non-CF.

Original languageEnglish (US)
Pages (from-to)963-968
Number of pages6
JournalJournal of Applied Physiology
Volume103
Issue number3
DOIs
StatePublished - Sep 2007

Fingerprint

Cystic Fibrosis
Vasodilation
Cholinergic Agents
Hot Temperature
Skin
Atropine
Laser-Doppler Flowmetry
Vasoactive Intestinal Peptide
Arterial Pressure
Iontophoresis
Cholinergic Receptors
Muscarinic Receptors
Blood Vessels
Blood Pressure
Neurons

Keywords

  • Acetylcholine
  • Human
  • Skin blood flow
  • Thermoregulation
  • Vasoactive intestinal peptide

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Cholinergic mechanisms of cutaneous active vasodilation during heat stress in cystic fibrosis. / Kellogg Jr, Dean L; Hodges, G. J.; Orozco, C. R.; Phillips, T. M.; Zhao, J. L.; Johnson, J. M.

In: Journal of Applied Physiology, Vol. 103, No. 3, 09.2007, p. 963-968.

Research output: Contribution to journalArticle

Kellogg Jr, Dean L ; Hodges, G. J. ; Orozco, C. R. ; Phillips, T. M. ; Zhao, J. L. ; Johnson, J. M. / Cholinergic mechanisms of cutaneous active vasodilation during heat stress in cystic fibrosis. In: Journal of Applied Physiology. 2007 ; Vol. 103, No. 3. pp. 963-968.
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