Cholesterol immobilization via ether-linked sepharose gels

Rajiv K. Satsangi, Glen E. Mott

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Cholesterol has been immobilized on Sepharose-6B via oxyether linkages to the 3- or 25-position. The 3- or 25-hydroxysterol methanesulfonates were coupled with epoxy-Sepharose-6B at 80 °C for 24 h. Approximately 2% of the ligand was incorporated into the gel. These types of affinity columns may be useful in purifying proteins that specifically bind or metabolize cholesterol.

Original languageEnglish (US)
Pages (from-to)953-957
Number of pages5
JournalThe Analyst
Issue number6
StatePublished - 1992
Externally publishedYes


  • 3β-tert-butyldimethylsilyloxycholest-5-en-25-one
  • 3β-tert-butyldimethylsilyloxycholest-5-en-25-yl methanesulfonate
  • 3β-tert-butyldimethylsilyloxychotest- 5-en-25-one
  • Cholesterol linked affinity gels

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry


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