Cholesterol has been immobilized on Sepharose-6B via oxyether linkages to the 3- or 25-position. The 3- or 25-hydroxysterol methanesulfonates were coupled with epoxy-Sepharose-6B at 80 °C for 24 h. Approximately 2% of the ligand was incorporated into the gel. These types of affinity columns may be useful in purifying proteins that specifically bind or metabolize cholesterol.
- 3β-tert-butyldimethylsilyloxycholest-5-en-25-yl methanesulfonate
- 3β-tert-butyldimethylsilyloxychotest- 5-en-25-one
- Cholesterol linked affinity gels
ASJC Scopus subject areas
- Analytical Chemistry
- Environmental Chemistry