Chlamydial protease-like activity factor mediated protection against C. trachomatis in guinea pigs

Shradha Wali, Rishein Gupta, Jieh Juen Yu, Gopala Krishna Koundinya Lanka, James P. Chambers, M. Neal Guentzel, Guangming Zhong, Ashlesh K. Murthy, Bernard P. Arulanandam

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We have comprehensively demonstrated using the mouse model that intranasal immunization with recombinant chlamydial protease-like activity factor (rCPAF) leads to a significant reduction in bacterial burden, genital tract pathology and preserves fertility following intravaginal genital chlamydial challenge. In the present report, we evaluated the protective efficacy of rCPAF immunization in guinea pigs, a second animal model for genital chlamydial infection. Using a vaccination strategy similar to the mouse model, we intranasally immunized female guinea pigs with rCPAF plus CpG deoxynucleotides (CpG; as an adjuvant), and challenged intravaginally with C. trachomatis serovar D (CT-D). Immunization with rCPAF/CpG significantly reduced vaginal CT-D shedding and induced resolution of infection by day 24, compared with day 33 in CpG alone treated and challenged animals. Immunization induced robust anti-rCPAF serum IgG 2 weeks following the last immunization, and was sustained at a high-level 4 weeks post challenge. Upregulation of antigen-specific IFN-Î 3 gene expression was observed in rCPAF/CpG-vaccinated splenocytes. Importantly, a significant reduction in inflammation in the genital tissue in rCPAF/CpG-immunized guinea pigs compared with CpG-immunized animals was observed. Taken together, this study provides evidence of the protective efficacy of rCPAF as a vaccine candidate in a second animal model of genital chlamydial infection.

Original languageEnglish (US)
Pages (from-to)454-460
Number of pages7
JournalImmunology and Cell Biology
Volume95
Issue number5
DOIs
StatePublished - May 1 2017

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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