Chlamydial plasmid-encoded virulence factor Pgp3 neutralizes the antichlamydial activity of human cathelicidin LL-37

Shuping Hou, Xiaohua Dong, Zhangsheng Yang, Zhongyu Li, Quanzhong Liu, Guangming Zhong

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Chlamydia trachomatis infection in the lower genital tract can ascend to and cause pathologies in the upper genital tract, potentially leading to severe complications, such as tubal infertility. However, chlamydial organisms depleted of plasmid or deficient in the plasmid-encoded Pgp3 are attenuated in ascending infection and no longer are able to induce the upper genital tract pathologies, indicating a significant role of Pgp3 in chlamydial pathogenesis. We now report that C. trachomatis Pgp3 can neutralize the antichlamydial activity of human cathelicidin LL-37, a host antimicrobial peptide secreted by both genital tract epithelial cells and infiltrating neutrophils. Pgp3 bound to and formed stable complexes with LL-37. We further showed that the middle region of Pgp3 (Pgp3m) was responsible for both the binding to and neutralization of LL-37, suggesting that Pgp3m can be targeted for attenuating chlamydial pathogenicity or developed for blocking LL-37-involved non-genital-tract pathologies, such as rosacea and psoriasis. Thus, the current study has provided significant information for both understanding the mechanisms of chlamydial pathogenesis and developing novel therapeutic agents.

Original languageEnglish (US)
Pages (from-to)4701-4709
Number of pages9
JournalInfection and Immunity
Volume83
Issue number12
DOIs
StatePublished - 2015

Fingerprint

Virulence Factors
Human Activities
Plasmids
Chlamydia trachomatis
Pathology
Rosacea
Chlamydia Infections
Psoriasis
Infertility
Virulence
Neutrophils
Epithelial Cells
Peptides
Infection
CAP18 lipopolysaccharide-binding protein
Therapeutics

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Chlamydial plasmid-encoded virulence factor Pgp3 neutralizes the antichlamydial activity of human cathelicidin LL-37. / Hou, Shuping; Dong, Xiaohua; Yang, Zhangsheng; Li, Zhongyu; Liu, Quanzhong; Zhong, Guangming.

In: Infection and Immunity, Vol. 83, No. 12, 2015, p. 4701-4709.

Research output: Contribution to journalArticle

Hou, Shuping ; Dong, Xiaohua ; Yang, Zhangsheng ; Li, Zhongyu ; Liu, Quanzhong ; Zhong, Guangming. / Chlamydial plasmid-encoded virulence factor Pgp3 neutralizes the antichlamydial activity of human cathelicidin LL-37. In: Infection and Immunity. 2015 ; Vol. 83, No. 12. pp. 4701-4709.
@article{ae2258296fb844a687eaff26f87dba37,
title = "Chlamydial plasmid-encoded virulence factor Pgp3 neutralizes the antichlamydial activity of human cathelicidin LL-37",
abstract = "Chlamydia trachomatis infection in the lower genital tract can ascend to and cause pathologies in the upper genital tract, potentially leading to severe complications, such as tubal infertility. However, chlamydial organisms depleted of plasmid or deficient in the plasmid-encoded Pgp3 are attenuated in ascending infection and no longer are able to induce the upper genital tract pathologies, indicating a significant role of Pgp3 in chlamydial pathogenesis. We now report that C. trachomatis Pgp3 can neutralize the antichlamydial activity of human cathelicidin LL-37, a host antimicrobial peptide secreted by both genital tract epithelial cells and infiltrating neutrophils. Pgp3 bound to and formed stable complexes with LL-37. We further showed that the middle region of Pgp3 (Pgp3m) was responsible for both the binding to and neutralization of LL-37, suggesting that Pgp3m can be targeted for attenuating chlamydial pathogenicity or developed for blocking LL-37-involved non-genital-tract pathologies, such as rosacea and psoriasis. Thus, the current study has provided significant information for both understanding the mechanisms of chlamydial pathogenesis and developing novel therapeutic agents.",
author = "Shuping Hou and Xiaohua Dong and Zhangsheng Yang and Zhongyu Li and Quanzhong Liu and Guangming Zhong",
year = "2015",
doi = "10.1128/IAI.00746-15",
language = "English (US)",
volume = "83",
pages = "4701--4709",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - Chlamydial plasmid-encoded virulence factor Pgp3 neutralizes the antichlamydial activity of human cathelicidin LL-37

AU - Hou, Shuping

AU - Dong, Xiaohua

AU - Yang, Zhangsheng

AU - Li, Zhongyu

AU - Liu, Quanzhong

AU - Zhong, Guangming

PY - 2015

Y1 - 2015

N2 - Chlamydia trachomatis infection in the lower genital tract can ascend to and cause pathologies in the upper genital tract, potentially leading to severe complications, such as tubal infertility. However, chlamydial organisms depleted of plasmid or deficient in the plasmid-encoded Pgp3 are attenuated in ascending infection and no longer are able to induce the upper genital tract pathologies, indicating a significant role of Pgp3 in chlamydial pathogenesis. We now report that C. trachomatis Pgp3 can neutralize the antichlamydial activity of human cathelicidin LL-37, a host antimicrobial peptide secreted by both genital tract epithelial cells and infiltrating neutrophils. Pgp3 bound to and formed stable complexes with LL-37. We further showed that the middle region of Pgp3 (Pgp3m) was responsible for both the binding to and neutralization of LL-37, suggesting that Pgp3m can be targeted for attenuating chlamydial pathogenicity or developed for blocking LL-37-involved non-genital-tract pathologies, such as rosacea and psoriasis. Thus, the current study has provided significant information for both understanding the mechanisms of chlamydial pathogenesis and developing novel therapeutic agents.

AB - Chlamydia trachomatis infection in the lower genital tract can ascend to and cause pathologies in the upper genital tract, potentially leading to severe complications, such as tubal infertility. However, chlamydial organisms depleted of plasmid or deficient in the plasmid-encoded Pgp3 are attenuated in ascending infection and no longer are able to induce the upper genital tract pathologies, indicating a significant role of Pgp3 in chlamydial pathogenesis. We now report that C. trachomatis Pgp3 can neutralize the antichlamydial activity of human cathelicidin LL-37, a host antimicrobial peptide secreted by both genital tract epithelial cells and infiltrating neutrophils. Pgp3 bound to and formed stable complexes with LL-37. We further showed that the middle region of Pgp3 (Pgp3m) was responsible for both the binding to and neutralization of LL-37, suggesting that Pgp3m can be targeted for attenuating chlamydial pathogenicity or developed for blocking LL-37-involved non-genital-tract pathologies, such as rosacea and psoriasis. Thus, the current study has provided significant information for both understanding the mechanisms of chlamydial pathogenesis and developing novel therapeutic agents.

UR - http://www.scopus.com/inward/record.url?scp=84949640632&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949640632&partnerID=8YFLogxK

U2 - 10.1128/IAI.00746-15

DO - 10.1128/IAI.00746-15

M3 - Article

C2 - 26416907

AN - SCOPUS:84949640632

VL - 83

SP - 4701

EP - 4709

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 12

ER -