Chlamydia trachomatis GlgA Is Secreted into Host Cell Cytoplasm

Chunxue Lu, Lei Lei, Bo Peng, Lingli Tang, Honglei Ding, Siqi Gong, Zhongyu Li, Yimou Wu, Guangming Zhong

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Glycogen has been localized both inside and outside Chlamydia trachomatis organisms. We now report that C. trachomatis glycogen synthase (GlgA) was detected in both chlamydial organism-associated and -free forms. The organism-free GlgA molecules were localized both in the lumen of chlamydial inclusions and in the cytosol of host cells. The cytosolic GlgA displayed a distribution pattern similar to that of a known C. trachomatis-secreted protease, CPAF. The detection of GlgA was specific since the anti-GlgA antibody labeling was only removed by preabsorption with GlgA but not CPAF fusion proteins. GlgA was detectable at 12h and its localization into host cell cytosol only became apparent at 24h after infection. The cytosolic localization of GlgA was conserved among all C. serovars. However, the significance of the GlgA secretion into host cell cytoplasm remains unclear since, while expression of chlamydial GlgA in HeLa cells increased glycogen stores, it did not affect a subsequent infection with C. trachomatis. Similar to several other C. trachomatis-secreted proteins, GlgA is immunogenic in women urogenitally infected with C. trachomatis, suggesting that GlgA is expressed and may be secreted into host cell cytosol during C. trachomatis infection in humans. These findings have provided important information for further understanding C. trachomatis pathogenic mechanisms.

Original languageEnglish (US)
Article numbere68764
JournalPLoS One
Volume8
Issue number7
DOIs
StatePublished - Jul 24 2013

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Chlamydia trachomatis
Glycogen
Cytoplasm
cytoplasm
Glycogen Synthase
Labeling
Anti-Idiotypic Antibodies
Proteins
Peptide Hydrolases
Fusion reactions
cytosol
cells
Cytosol
Molecules
glycogen
glycogen (starch) synthase
organisms
infection
Chlamydia Infections
Infection

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Chlamydia trachomatis GlgA Is Secreted into Host Cell Cytoplasm. / Lu, Chunxue; Lei, Lei; Peng, Bo; Tang, Lingli; Ding, Honglei; Gong, Siqi; Li, Zhongyu; Wu, Yimou; Zhong, Guangming.

In: PLoS One, Vol. 8, No. 7, e68764, 24.07.2013.

Research output: Contribution to journalArticle

Lu, C, Lei, L, Peng, B, Tang, L, Ding, H, Gong, S, Li, Z, Wu, Y & Zhong, G 2013, 'Chlamydia trachomatis GlgA Is Secreted into Host Cell Cytoplasm', PLoS One, vol. 8, no. 7, e68764. https://doi.org/10.1371/journal.pone.0068764
Lu C, Lei L, Peng B, Tang L, Ding H, Gong S et al. Chlamydia trachomatis GlgA Is Secreted into Host Cell Cytoplasm. PLoS One. 2013 Jul 24;8(7). e68764. https://doi.org/10.1371/journal.pone.0068764
Lu, Chunxue ; Lei, Lei ; Peng, Bo ; Tang, Lingli ; Ding, Honglei ; Gong, Siqi ; Li, Zhongyu ; Wu, Yimou ; Zhong, Guangming. / Chlamydia trachomatis GlgA Is Secreted into Host Cell Cytoplasm. In: PLoS One. 2013 ; Vol. 8, No. 7.
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