Chlamydia trachomatis antigens recognized in women with tubal factor infertility, normal fertility, and acute infection

Nicole M. Budrys, Siqi Gong, Allison K. Rodgers, Jie Wang, Christopher Louden, Rochelle Shain, Robert S Schenken, Guangming Zhong

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

OBJECTIVE:: To identify Chlamydia trachomatis antigens associated with tubal factor infertility and acute infection. METHODS:: A C trachomatis proteome array was used to compare antibody profiles among women with tubal factor infertility, normal fertility, and acute C trachomatis infection. RESULTS:: Thirteen immunodominant antigens reacted with 50% or more sera from all women (n=73). Six C trachomatis antigens were uniquely recognized in women with tubal factor infertility. Combining fragmentation of the six antigens with serum sample dilution, chlamydial antigens HSP60, CT376, CT557, and CT443 could discriminate between women with tubal factor infertility and women with normal fertility with a sensitivity of 63% (95% confidence interval [CI] 0.41-0.77) and specificity of 100% (95% CI 0.91-1), respectively. These antigens were designated as tubal factor infertility-associated antigens. However, these tubal factor antigens were unable to distinguish tubal factor infertility patients from those with acute infection. A combination of CT875 and CT147 distinguished women with acute infection from all other C trachomatis-exposed women with a detection sensitivity of 63% (95% CI 0.41-0.77) and specificity of 100% (95% CI 0.95-1), respectively. Thus, CT875 and CT147 were designated as acute infection-associated antigens. CONCLUSION:: A sequential screening of antibodies against panels of C trachomatis antigens can be used to identify women with tubal factor infertility and acute C trachomatis infection.

Original languageEnglish (US)
Pages (from-to)1009-1016
Number of pages8
JournalObstetrics and Gynecology
Volume119
Issue number5
DOIs
StatePublished - May 2012

Fingerprint

Chlamydia trachomatis
Infertility
Fertility
Antigens
Infection
Confidence Intervals
Immunodominant Epitopes
Antibodies
Proteome
Serum

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Chlamydia trachomatis antigens recognized in women with tubal factor infertility, normal fertility, and acute infection. / Budrys, Nicole M.; Gong, Siqi; Rodgers, Allison K.; Wang, Jie; Louden, Christopher; Shain, Rochelle; Schenken, Robert S; Zhong, Guangming.

In: Obstetrics and Gynecology, Vol. 119, No. 5, 05.2012, p. 1009-1016.

Research output: Contribution to journalArticle

Budrys, Nicole M. ; Gong, Siqi ; Rodgers, Allison K. ; Wang, Jie ; Louden, Christopher ; Shain, Rochelle ; Schenken, Robert S ; Zhong, Guangming. / Chlamydia trachomatis antigens recognized in women with tubal factor infertility, normal fertility, and acute infection. In: Obstetrics and Gynecology. 2012 ; Vol. 119, No. 5. pp. 1009-1016.
@article{58dc756994524590b0aa05c8664c9006,
title = "Chlamydia trachomatis antigens recognized in women with tubal factor infertility, normal fertility, and acute infection",
abstract = "OBJECTIVE:: To identify Chlamydia trachomatis antigens associated with tubal factor infertility and acute infection. METHODS:: A C trachomatis proteome array was used to compare antibody profiles among women with tubal factor infertility, normal fertility, and acute C trachomatis infection. RESULTS:: Thirteen immunodominant antigens reacted with 50{\%} or more sera from all women (n=73). Six C trachomatis antigens were uniquely recognized in women with tubal factor infertility. Combining fragmentation of the six antigens with serum sample dilution, chlamydial antigens HSP60, CT376, CT557, and CT443 could discriminate between women with tubal factor infertility and women with normal fertility with a sensitivity of 63{\%} (95{\%} confidence interval [CI] 0.41-0.77) and specificity of 100{\%} (95{\%} CI 0.91-1), respectively. These antigens were designated as tubal factor infertility-associated antigens. However, these tubal factor antigens were unable to distinguish tubal factor infertility patients from those with acute infection. A combination of CT875 and CT147 distinguished women with acute infection from all other C trachomatis-exposed women with a detection sensitivity of 63{\%} (95{\%} CI 0.41-0.77) and specificity of 100{\%} (95{\%} CI 0.95-1), respectively. Thus, CT875 and CT147 were designated as acute infection-associated antigens. CONCLUSION:: A sequential screening of antibodies against panels of C trachomatis antigens can be used to identify women with tubal factor infertility and acute C trachomatis infection.",
author = "Budrys, {Nicole M.} and Siqi Gong and Rodgers, {Allison K.} and Jie Wang and Christopher Louden and Rochelle Shain and Schenken, {Robert S} and Guangming Zhong",
year = "2012",
month = "5",
doi = "10.1097/AOG.0b013e3182519326",
language = "English (US)",
volume = "119",
pages = "1009--1016",
journal = "Obstetrics and Gynecology",
issn = "0029-7844",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Chlamydia trachomatis antigens recognized in women with tubal factor infertility, normal fertility, and acute infection

AU - Budrys, Nicole M.

AU - Gong, Siqi

AU - Rodgers, Allison K.

AU - Wang, Jie

AU - Louden, Christopher

AU - Shain, Rochelle

AU - Schenken, Robert S

AU - Zhong, Guangming

PY - 2012/5

Y1 - 2012/5

N2 - OBJECTIVE:: To identify Chlamydia trachomatis antigens associated with tubal factor infertility and acute infection. METHODS:: A C trachomatis proteome array was used to compare antibody profiles among women with tubal factor infertility, normal fertility, and acute C trachomatis infection. RESULTS:: Thirteen immunodominant antigens reacted with 50% or more sera from all women (n=73). Six C trachomatis antigens were uniquely recognized in women with tubal factor infertility. Combining fragmentation of the six antigens with serum sample dilution, chlamydial antigens HSP60, CT376, CT557, and CT443 could discriminate between women with tubal factor infertility and women with normal fertility with a sensitivity of 63% (95% confidence interval [CI] 0.41-0.77) and specificity of 100% (95% CI 0.91-1), respectively. These antigens were designated as tubal factor infertility-associated antigens. However, these tubal factor antigens were unable to distinguish tubal factor infertility patients from those with acute infection. A combination of CT875 and CT147 distinguished women with acute infection from all other C trachomatis-exposed women with a detection sensitivity of 63% (95% CI 0.41-0.77) and specificity of 100% (95% CI 0.95-1), respectively. Thus, CT875 and CT147 were designated as acute infection-associated antigens. CONCLUSION:: A sequential screening of antibodies against panels of C trachomatis antigens can be used to identify women with tubal factor infertility and acute C trachomatis infection.

AB - OBJECTIVE:: To identify Chlamydia trachomatis antigens associated with tubal factor infertility and acute infection. METHODS:: A C trachomatis proteome array was used to compare antibody profiles among women with tubal factor infertility, normal fertility, and acute C trachomatis infection. RESULTS:: Thirteen immunodominant antigens reacted with 50% or more sera from all women (n=73). Six C trachomatis antigens were uniquely recognized in women with tubal factor infertility. Combining fragmentation of the six antigens with serum sample dilution, chlamydial antigens HSP60, CT376, CT557, and CT443 could discriminate between women with tubal factor infertility and women with normal fertility with a sensitivity of 63% (95% confidence interval [CI] 0.41-0.77) and specificity of 100% (95% CI 0.91-1), respectively. These antigens were designated as tubal factor infertility-associated antigens. However, these tubal factor antigens were unable to distinguish tubal factor infertility patients from those with acute infection. A combination of CT875 and CT147 distinguished women with acute infection from all other C trachomatis-exposed women with a detection sensitivity of 63% (95% CI 0.41-0.77) and specificity of 100% (95% CI 0.95-1), respectively. Thus, CT875 and CT147 were designated as acute infection-associated antigens. CONCLUSION:: A sequential screening of antibodies against panels of C trachomatis antigens can be used to identify women with tubal factor infertility and acute C trachomatis infection.

UR - http://www.scopus.com/inward/record.url?scp=84860187238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860187238&partnerID=8YFLogxK

U2 - 10.1097/AOG.0b013e3182519326

DO - 10.1097/AOG.0b013e3182519326

M3 - Article

C2 - 22525912

AN - SCOPUS:84860187238

VL - 119

SP - 1009

EP - 1016

JO - Obstetrics and Gynecology

JF - Obstetrics and Gynecology

SN - 0029-7844

IS - 5

ER -