Chemopreventive effects of Khaya senegalensis bark extract on human colorectal cancer

Xiao M. Androulakis; Stephanie J. Muga; Feng Chen; Youssouf Koita; Boubacar Toure; Michael J. Wargovich

Chemopreventive effects of Khaya senegalensis bark extract on human colorectal cancer

Xiao M. Androulakis, Stephanie J. Muga, Feng Chen, Youssouf Koita, Boubacar Toure, Michael J. Wargovich

Research output: Contribution to journal › Article › peer-review

Abstract

An extract of the bark of Khaya senegalensis is commonly used in African traditional medicine for pain and inflammation. Khaya senegalensis bark extract (KSBE) was hypothesized to contain inhibitors of the cyclooxygenase-2 (COX-2) gene and to be useful in the prevention and treatment of colorectal cancer. The diphenyl-2-picrylhydrazyl (DPPH)-free radical activity and the total phenolic content of KSBE were measured, followed by an investigation of cell growth inhibition, COX and prostaglandin E 2 (PGE₂) suppression, as well as apoptosis by Western blot analysis and ELISA. Our data clearly showed that KSBE displays anti-proliferative, antiinflammatory and pro-apoptotic effects on HT-29, HCT-15 and HCA-7 cells. Since all three cell lines, irrespective of COX-2 status (HCT-15 is COX-2-deficient), were affected by the treatment, it can be concluded that both COX-dependent and COX-independent pathways are activated by KSBE.

Original language	English (US)
Pages (from-to)	2397-2405
Number of pages	9
Journal	Anticancer Research
Volume	26
Issue number	3 B
State	Published - May 2006
Externally published	Yes

Keywords

COX-2
Caspase 3
Cell cycle
Khaya senegalensis
PGE

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

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title = "Chemopreventive effects of Khaya senegalensis bark extract on human colorectal cancer",

abstract = "An extract of the bark of Khaya senegalensis is commonly used in African traditional medicine for pain and inflammation. Khaya senegalensis bark extract (KSBE) was hypothesized to contain inhibitors of the cyclooxygenase-2 (COX-2) gene and to be useful in the prevention and treatment of colorectal cancer. The diphenyl-2-picrylhydrazyl (DPPH)-free radical activity and the total phenolic content of KSBE were measured, followed by an investigation of cell growth inhibition, COX and prostaglandin E 2 (PGE2) suppression, as well as apoptosis by Western blot analysis and ELISA. Our data clearly showed that KSBE displays anti-proliferative, antiinflammatory and pro-apoptotic effects on HT-29, HCT-15 and HCA-7 cells. Since all three cell lines, irrespective of COX-2 status (HCT-15 is COX-2-deficient), were affected by the treatment, it can be concluded that both COX-dependent and COX-independent pathways are activated by KSBE.",

keywords = "COX-2, Caspase 3, Cell cycle, Khaya senegalensis, PGE",

author = "Androulakis, {Xiao M.} and Muga, {Stephanie J.} and Feng Chen and Youssouf Koita and Boubacar Toure and Wargovich, {Michael J.}",

year = "2006",

month = may,

language = "English (US)",

volume = "26",

pages = "2397--2405",

journal = "Anticancer Research",

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publisher = "International Institute of Anticancer Research",

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}

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T1 - Chemopreventive effects of Khaya senegalensis bark extract on human colorectal cancer

AU - Androulakis, Xiao M.

AU - Muga, Stephanie J.

AU - Chen, Feng

AU - Koita, Youssouf

AU - Toure, Boubacar

AU - Wargovich, Michael J.

PY - 2006/5

Y1 - 2006/5

N2 - An extract of the bark of Khaya senegalensis is commonly used in African traditional medicine for pain and inflammation. Khaya senegalensis bark extract (KSBE) was hypothesized to contain inhibitors of the cyclooxygenase-2 (COX-2) gene and to be useful in the prevention and treatment of colorectal cancer. The diphenyl-2-picrylhydrazyl (DPPH)-free radical activity and the total phenolic content of KSBE were measured, followed by an investigation of cell growth inhibition, COX and prostaglandin E 2 (PGE2) suppression, as well as apoptosis by Western blot analysis and ELISA. Our data clearly showed that KSBE displays anti-proliferative, antiinflammatory and pro-apoptotic effects on HT-29, HCT-15 and HCA-7 cells. Since all three cell lines, irrespective of COX-2 status (HCT-15 is COX-2-deficient), were affected by the treatment, it can be concluded that both COX-dependent and COX-independent pathways are activated by KSBE.

AB - An extract of the bark of Khaya senegalensis is commonly used in African traditional medicine for pain and inflammation. Khaya senegalensis bark extract (KSBE) was hypothesized to contain inhibitors of the cyclooxygenase-2 (COX-2) gene and to be useful in the prevention and treatment of colorectal cancer. The diphenyl-2-picrylhydrazyl (DPPH)-free radical activity and the total phenolic content of KSBE were measured, followed by an investigation of cell growth inhibition, COX and prostaglandin E 2 (PGE2) suppression, as well as apoptosis by Western blot analysis and ELISA. Our data clearly showed that KSBE displays anti-proliferative, antiinflammatory and pro-apoptotic effects on HT-29, HCT-15 and HCA-7 cells. Since all three cell lines, irrespective of COX-2 status (HCT-15 is COX-2-deficient), were affected by the treatment, it can be concluded that both COX-dependent and COX-independent pathways are activated by KSBE.

KW - COX-2

KW - Caspase 3

KW - Cell cycle

KW - Khaya senegalensis

KW - PGE

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SN - 0250-7005

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JO - Anticancer Research

JF - Anticancer Research

IS - 3 B

ER -

Chemopreventive effects of Khaya senegalensis bark extract on human colorectal cancer

Abstract

Keywords

ASJC Scopus subject areas

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