Chemical characterization and biologic properties of lipopolysaccharide from Bacteroides gingivalis strains W50, W83, and ATCC 33277

Thomas E. Bramanti, Guie G. Wong, Susan T. Weintraub, Stanley C. Holt

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

The chemistry and selected biological activity of lipopolysaccharide (LPS) from Bacteroides gingivalis strains W50, W83, and ATCC 33277 were compared, as well as the role of this molecule as a mediator of selected inflammatory responses. Chemically, the LPSs consisted of 47–58% Lipid A, 5–10% carbohydrate, 0.05% 3‐deoxy 2‐octulosonic acid, 0.3% heptose, 3.8–5.2% hexosamine, and 2% phosphate. Rhamnose represented the dominant sugar (26–36%), with lesser amounts of glucose (18–34%), galactose (18–25%), mannose (9–12%), glucosamine (7–11%), and galaclosamine (2–5%). The major fatty acids were: 13‐methyl‐tetradecanoate (42–45%), 3‐OH‐heptadecanoate (21–23%), hexadecanoate (16–19%), and 12‐methyl‐tetradecanoate (6–8%). SDS‐PAGE and sodium deoxy‐cholate‐PAGE revealed the LPS to be a smooth chemotype. Differences in migration patterns between the virulent and avirulent strain LPSs also occurred. C3H/HeN macrophages (Mo) exposed to 1 μg/ml of LPS released 3.2–4.2 ng of prostaglandin E (PGE)/ml of supernatant, representing 236–278% of control. Interleukin‐1 (IL‐1) activity in C3H/HeN and C3H/HeJ M0 exposed to 50 /*g of LPS/ml was 382–724% and 270–300% of control, respectively; similar Mo exposed to 10 μg of LPS/ml released 1.6–2.0 ng and 0.3–0.5 ng of tumor necrosis factor (TNF)/ml of supernatant, respectively. Maximum TNF release in C3H/HeN M0 occurred in response to 50 μg of LPS/ml, and was sustained for up to 96 hours. These results suggest that LPS from the B. gingivalis strains stimulate cytokine production from M0 which, in turn, may play a role in orchestrating the inflammatory response for the development of periodontal diseases.

Original languageEnglish (US)
Pages (from-to)183-192
Number of pages10
JournalOral Microbiology and Immunology
Volume4
Issue number4
DOIs
StatePublished - Aug 1989

Keywords

  • Bacteroides gingivalis
  • LPS
  • endotoxin
  • interleukin I
  • outer membrane
  • outer membrane vesicle
  • prostoglandin
  • tumor necrosis factor

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)

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