Characterizing the Electron Transport Chain: Structural Approach

Ting Liang, Janice Deng, Bijaya Nayak, Xin Zou, Yuji Ikeno, Yidong Bai

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The mitochondrial respiratory chain which carries out the oxidative phosphorylation (OXPHOS) consists of five multi-subunit protein complexes. Emerging evidences suggest that the supercomplexes which further consist of multiple respiratory complexes play important role in regulating OXPHOS function. Dysfunction of the respiratory chain and its regulation has been implicated in various human diseases including neurodegenerative diseases and muscular disorders. Many mouse models have been established which exhibit mitochondrial defects in brain and muscles. Protocols presented here aim to help to analyze the structures of mitochondrial respiratory chain which include the preparation of the tissue samples, isolation of mitochondrial membrane proteins, and analysis of their respiratory complexes by Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) in particular.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages107-115
Number of pages9
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2497
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Assembly
  • Blue Native Gel
  • Brain
  • Muscle
  • Respiratory complex

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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