@inbook{07beb027c0a848a1bd4f94c593569d9b,
title = "Characterizing the Electron Transport Chain: Functional Approach Using Extracellular Flux Analyzer on Mouse Tissue Samples",
abstract = "The Seahorse Extracellular Flux Analyzer enables the high-throughput characterization of oxidative phosphorylation capacity based on the electron transport chain organization and regulation with relatively small amount of material. This development over the traditional polarographic Clark-type electrode approaches make it possible to analyze the respiratory features of mitochondria isolated from tissue samples of particular animal models. Here we provide a description of an optimized approach to carry out multi-well measurement of O2 consumption, with the Agilent Seahorse XFe96 analyzer on mouse brain and muscles to determine the tissue-specific oxidative phosphorylation properties. Protocols include the preparation of the tissue samples, isolation of mitochondria, and analysis of their function; in particular, the preparation and optimization of the reagents and samples.",
keywords = "Brain, Electron transport chain, Muscle, O consumption, Seahorse",
author = "Ting Liang and Jay Dunn and Xin Zou and Bijaya Nayak and Yuji Ikeno and Lihong Fan and Yidong Bai",
note = "Funding Information: This work is supported by grants from National Institute of Health (R01 GM109434 and GM130129), the Pathology Core in the San Antonio Nathan Shock Center (P30-AG013319), and LT and YB are also supported by William and Ella Owens Medical Research Foundation. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2309-1_8",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "117--128",
booktitle = "Methods in Molecular Biology",
address = "United States",
}