Characterizing and Tuning Perfusion Parameters Within an Innovative, Versatile Oxygenating Perfusion System

Daniel J. Portillo, Lauren Bayliss, Stephen Rivas, Gabriela Pineda, Sukhwinder Kaur, Leonid Bunegin, R. Lyle Hood

Research output: Contribution to journalArticlepeer-review

Abstract

The advantages of oxygenated perfusion are continuing to be demonstrated by many groups focused on improving the efficacy of tissue preservation for transplant, bioreactors for studies of basic tissue physiology, and closed-loop resuscitation. This work presents a novel and portable device that supplies oxygenated and pulsatile perfusion, both of which are regulated by a single pump-oxygenator component comprised of silicone tubes that are cyclically inflated/deflated with compressed oxygen. In this study, pump variables (oxygen supply pressure and length of a silicone tube) were evaluated against hydraulic elements that mimicked the vascular resistance of kidneys, livers, and hearts. The perfusion pressures, flow rates, and oxygenation rates produced by the device were characterized for all configurations of pump variables, and the pulse rates were tuned to improve performance. The device supplied perfusion pressures ranging from 3.5 to 109 mmHg, flow rates ranging from 1.4 to 71.8 mL min−1, and oxygenation rates up to 316.6 µmol min−1. From those results, it was determined that the device was capable of achieving perfusion parameters used in previous kidney, liver, and heart preservation studies. Ultimately, this research demonstrated the efficacy of a novel device that is designed to supply oxygenated perfusion across a range of applications.

Original languageEnglish (US)
Pages (from-to)3154-3164
Number of pages11
JournalAnnals of Biomedical Engineering
Volume49
Issue number12
DOIs
StatePublished - Dec 2021
Externally publishedYes

Keywords

  • Bioreactor
  • Closed-loop resuscitation
  • Heart preservation
  • Kidney preservation
  • Liver preservation
  • Machine perfusion
  • Organ preservation
  • Tissue preservation

ASJC Scopus subject areas

  • Biomedical Engineering

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